{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:10:20Z","timestamp":1787962220087,"version":"build-2784847793"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T00:00:00Z","timestamp":1673395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100013076","name":"National Major Science and Technology Projects of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013076","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Invasive pulmonary aspergillosis (IPA) can occur in both immunocompromised and non\u2010immunocompromised hosts, and early diagnosis of IPA is difficult. Metagenomic next\u2010generation sequencing (mNGS) is a novel non\u2010migratory pathogen detection method; however, utilising this method for IPA diagnosis is challenging due to the current lack of a unified clinical interpretation standard following <jats:italic>Aspergillus<\/jats:italic> detection using mNGS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate the accuracy of IPA diagnosis by positive bronchoalveolar lavage fluid (BALF) mNGS results in immunocompromised and immunocompetent patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We retrospectively included patients with confirmed pulmonary infections having a BALF mNGS result of <jats:italic>Aspergillus<\/jats:italic> reads \u22651. We compared the accuracy of using mNGS for IPA diagnosis in patients with different immune statuses based on the revised EORTC\/MSG criteria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, 62 mNGS <jats:italic>Aspergillus<\/jats:italic>\u2010positive patients were divided into two groups: with (41) and without IPA (21). In univariate logistic regression analysis, immunocompromised function, fever, halo sign on CT image, and multiple masses or nodules were associated with mNGS <jats:italic>Aspergillus<\/jats:italic>\u2010positive IPA diagnosis. In multivariate logistic regression analysis, immunocompromised function (OR\u00a0=\u00a06.68, 95% CI: 1.73\u201325.87, <jats:italic>p<\/jats:italic>\u00a0=\u00a0.006) and a halo sign (OR\u00a0=\u00a07.993, 95% CI: 2.07\u201330.40, <jats:italic>p<\/jats:italic>\u00a0=\u00a0.003) were independent risk factors. The concordance rate of IPA diagnosis was significantly higher in immunocompromised patients [82.1% (23\/28)] than in non\u2010immunocompromised patients [52.9% (18\/34); <jats:italic>p<\/jats:italic>\u00a0=\u00a0.016].<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>For immunocompromised patients, a combination of mNGS testing and lung CT imaging can be used for IPA diagnosis. However, caution is required in IPA diagnosis based on positive mNGS results in non\u2010immunocompromised patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13557","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T08:12:20Z","timestamp":1671610340000},"page":"331-337","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Evaluation of metagenomic next\u2010generation sequencing diagnosis for invasive pulmonary aspergillosis in immunocompromised and immunocompetent patients"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6538-555X","authenticated-orcid":false,"given":"Wenyu","family":"Zhan","sequence":"first","affiliation":[{"name":"Respiratory Department Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingjun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Geriatrics The Second Hospital of Tianjin Medical University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changqing","family":"Yang","sequence":"additional","affiliation":[{"name":"Respiratory Department Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Respiratory Department Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[{"name":"Respiratory Department Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yubao","family":"Wang","sequence":"additional","affiliation":[{"name":"Respiratory Department Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Feng","sequence":"additional","affiliation":[{"name":"Respiratory Department Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30309-2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2020.02.025"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-3401990"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12890-018-0638-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.02223-2020"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/resp.13032"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2014.07.011"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-1696968"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-020-01424-x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myy103"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-019-0113-7"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-1105-z"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1809700115"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2016.1235671"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2014-206291"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2020.05.598"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201202-0320ST"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy693"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0808853"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2015.10.015"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00140-18"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/723129"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof8050480"},{"issue":"4","key":"e_1_2_9_25_1","first-page":"TE01","article-title":"Pulmonary aspergillosis: what CT can offer before it is too late!","volume":"10","author":"Prasad A","year":"2016","journal-title":"J Clin Diagn Res"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1086\/509917"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.925982"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201111-1978OC"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2021.03.008"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myx011"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.24147"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0761-8417(07)90120-0"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(18)30274-1"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myy149"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.13557","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/myc.13557","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.13557","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T13:04:10Z","timestamp":1692277450000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.13557"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,11]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["10.1111\/myc.13557"],"URL":"https:\/\/doi.org\/10.1111\/myc.13557","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,11]]},"assertion":[{"value":"2022-10-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}