{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T07:03:25Z","timestamp":1792134205127,"version":"4.4.0"},"reference-count":71,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T00:00:00Z","timestamp":1679875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Sporotrichosis is an implantation mycosis caused by the dimorphic fungus\n                    <jats:italic>Sporothrix<\/jats:italic>\n                    and mostly involves cutaneous and subcutaneous tissues and the lymphatic vessels. Among more than 50 different species, only\n                    <jats:italic>Sporothrix schenckii, Sporothrix globosa<\/jats:italic>\n                    and\n                    <jats:italic>Sporothrix brasiliensis<\/jats:italic>\n                    are frequently reported to cause infections in humans.\n                    <jats:italic>Sporothrix brasiliensis<\/jats:italic>\n                    is remarkably virulent and has been spreading rapidly in Brazil and other Latin American countries. In this study, we aimed to determine the genetic relatedness and antifungal susceptibility of\n                    <jats:italic>Sporothrix<\/jats:italic>\n                    strains by analysing 89 isolates from humans and cats in Curitiba, Southern Brazil. Calmodulin sequencing identified 81\u2009\n                    <jats:italic>S.\u2009brasiliensis<\/jats:italic>\n                    and seven\n                    <jats:italic>S.\u2009schenckii<\/jats:italic>\n                    isolates. Amplified fragment length polymorphism genotyping analysis showed feline and human isolates clustering together. In vitro susceptibility testing with seven antifungals demonstrated a broad activity against all tested\n                    <jats:italic>S.\u2009brasiliensis<\/jats:italic>\n                    isolates, with no significant differences in minimal inhibitory concentration (MIC) values between feline and human isolates. Resistance was solely observed in one human isolate against itraconazole and posaconazole, with MICs of \u226516\u2009\u03bcg\/mL against both antifungals. Whole genome sequencing (WGS) analysis on this isolate and two related susceptible isolates did not reveal any unique substitutions in resistance\u2010associated genes, including\n                    <jats:italic>cyp51<\/jats:italic>\n                    ,\n                    <jats:italic>hmg<\/jats:italic>\n                    and\n                    <jats:italic>erg6,<\/jats:italic>\n                    when compared to two related susceptible isolates. The novel antifungal olorofim exhibited excellent activity against this large isolate collection, with all isolates considered as susceptible. Altogether, we indicate zoonotic transmission based on genotyping and revealed a broad activity of seven common antifungals, including olorofim, against a large\n                    <jats:italic>S.\u2009brasiliensis<\/jats:italic>\n                    isolate collection.\n                  <\/jats:p>","DOI":"10.1111\/myc.13584","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T06:49:41Z","timestamp":1679899781000},"page":"585-593","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Genotyping and antifungal susceptibility testing of\n                    <i>Sporothrix brasiliensis<\/i>\n                    isolates from Southern Brazil"],"prefix":"10.1111","volume":"66","author":[{"given":"Amanda","family":"Bombassaro","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases Canisius\u2010Wilhelmina Hospital  Nijmegen The 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Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Graduation in Biological Sciences Federal University of Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna","family":"Jacomel\u00a0Favoreto\u00a0de\u00a0Souza\u00a0Lima","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases Canisius\u2010Wilhelmina Hospital  Nijmegen The Netherlands"},{"name":"Department of Basic Pathology, Graduate Program in Microbiology, Parasitology and Pathology, Biological Sciences Federal University of Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana Beatriz","family":"Ballardin","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science Catholic University of Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marconi Rodrigues de","family":"Farias","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science Catholic University of Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vania Aparecida","family":"Vicente","sequence":"additional","affiliation":[{"name":"Center of Expertise in Mycology Radboud University Medical Center\/Canisius\u2010Wilhelmina Hospital and Excellence Center for Medical Mycology  Nijmegen The Netherlands"},{"name":"Department of Bioprocess Engineering and Biotechnology, Engineering Bioprocess and Biotechnology Graduate Program Federal University of Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavio","family":"de Queiroz\u2010Telles","sequence":"additional","affiliation":[{"name":"Department of Basic Pathology, Graduate Program in Microbiology, Parasitology and Pathology, Biological 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