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Both panels included a range of concentrations of\n                      <jats:italic>H. capsulatum<\/jats:italic>\n                      (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20097) and\n                      <jats:italic>Coccidioides<\/jats:italic>\n                      spp. (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20096), negative control and DNA from other fungi. Four laboratories used specific qPCRs, and one laboratory a broad\u2010range fungal conventional PCR (cPCR) and a specific cPCR for\n                      <jats:italic>H. capsulatum<\/jats:italic>\n                      with subsequent sequencing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      qPCR assays were the most sensitive for the detection of\n                      <jats:italic>H. capsulatum<\/jats:italic>\n                      DNA. The lowest amount of\n                      <jats:italic>H. capsulatum<\/jats:italic>\n                      DNA detected was 1\u20134\u2009fg, 0.1\u2009pg and 10\u2009pg for qPCRs, specific cPCR and broad\u2010range cPCR, respectively. False positive results occurred with high concentrations of\n                      <jats:italic>Blastomyces dermatitidis<\/jats:italic>\n                      DNA in two laboratories and with\n                      <jats:italic>Emergomyces<\/jats:italic>\n                      spp. in one laboratory. For the\n                      <jats:italic>Coccidioides<\/jats:italic>\n                      panel, the lowest amount of DNA detected was 1\u201316\u2009fg by qPCRs and 10\u2009pg with the broad\u2010range cPCR. One laboratory reported a false positive result by qPCR with high load of\n                      <jats:italic>Uncinocarpus<\/jats:italic>\n                      DNA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      All five laboratories were able to correctly detect\n                      <jats:italic>H. capsulatum<\/jats:italic>\n                      and\n                      <jats:italic>Coccidioides<\/jats:italic>\n                      spp. DNA and qPCRs had a better performance than specific cPCR and broad\u2010range cPCR. EQAs may help standardise in\u2010house molecular tests for the so\u2010called endemic mycoses improving patient management.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.13603","type":"journal-article","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T22:55:00Z","timestamp":1683845700000},"page":"774-786","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A multicentre external quality assessment: A first step to standardise\n                    <scp>PCR<\/scp>\n                    protocols for the diagnosis of histoplasmosis and coccidioidomycosis"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5466-1248","authenticated-orcid":false,"given":"Dunja","family":"Wilmes","sequence":"first","affiliation":[{"name":"FG16, Unit for Mycotic and Parasitic Agents and Mycobacteria Robert Koch Institute  Berlin 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