{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:10:18Z","timestamp":1787501418564,"version":"build-2736575974"},"reference-count":19,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,7,11]],"date-time":"2023-07-11T00:00:00Z","timestamp":1689033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Dermatophytosis is one of the most common fungal infections worldwide. The distribution of dermatophytes varies across continents, but the genera\n                      <jats:italic>Trichophyton<\/jats:italic>\n                      and\n                      <jats:italic>Microsporum<\/jats:italic>\n                      have emerged as the main isolated agents in humans and animals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To validate\n                      <jats:italic>Drosophila melanogaster<\/jats:italic>\n                      flies as a fast and feasible model to study dermatophytic infections.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Wild\u2010type (WT) and\n                      <jats:italic>Toll<\/jats:italic>\n                      \u2010deficient\n                      <jats:italic>D.\u2009melanogaster<\/jats:italic>\n                      flies were infected by\n                      <jats:italic>Trichophyton rubrum<\/jats:italic>\n                      ,\n                      <jats:italic>T.\u2009mentagrophytes<\/jats:italic>\n                      ,\n                      <jats:italic>Microsporum canis<\/jats:italic>\n                      and\n                      <jats:italic>Nannizzia gypsea<\/jats:italic>\n                      by pricking with a needle previously dipped in inoculum concentrations ranging from 10\n                      <jats:sup>3<\/jats:sup>\n                      to 10\n                      <jats:sup>8<\/jats:sup>\n                      colony\u2010forming units\/mL. Establishment of infection was confirmed by survival curves, histopathological analysis and fungal burden. Thereafter, flies were treated with terbinafine, itraconazole and clioquinol.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      WT flies were predominantly resistant to the infection, whereas\n                      <jats:italic>Toll<\/jats:italic>\n                      \u2010deficient flies succumbed to the four dermatophyte genera tested. The antifungal drugs protected flies from the infection, except for\n                      <jats:italic>N.\u2009gypsea<\/jats:italic>\n                      whose survival curves did not differ from the untreated group.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This pilot study confirms that\n                      <jats:italic>D.\u2009melanogaster<\/jats:italic>\n                      is a suitable model to study the virulence and antifungal drug efficacy in dermatophyte species.\n                    <\/jats:p>\n                  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