{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:41:42Z","timestamp":1753879302918,"version":"3.41.2"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T00:00:00Z","timestamp":1697155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Paracoccidioidomycosis is the most prevalent systemic mycosis in Latin America, with a high incidence in Brazil, Colombia and Venezuela, and constitutes a serious public health problem, a frequent cause of morbidity and disability for work. Some mechanisms of cell death are described as important tools in infectious processes. When apoptosis is blocked, RIPK (<jats:italic>Receptor\u2010interacting protein kinase)<\/jats:italic> 3 dependent, a caspase\u2010independent form of cell death, can limit the replication and spread of pathogens. Some molecules that mediate necroptosis include RIPK3 and have been extensively studied due to their signalling mechanism and pathological function. RIPK3 activates NLRP1 and NLRP3\u2010mediated inflammasome formation. Caspase\u20101 has an important role in processing the cytokines IL\u03b2 and IL18 to their active form. Such molecules are part of the inflammasome characterization, whose caspase\u20101\u2010dependent activation promotes the death of pyroptotic cells and the secretion of proinflammatory cytokines. Knowledge about the mechanisms of pathogen\u2010mediated cell death can be useful for understanding of the pathogenesis of infections and inflammatory conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The objective of this work was to identify the mechanisms of programmed cell death and inflammasome components in human oral mucosal lesions of paracoccidioidomycosis through immunohistochemical methods and identification of RIPK\u20103, IL1\u03b2, IL18, NLRP\u20101 and caspase\u20101. Thirty specimens were included, and a histopathological analysis of the lesions was performed using haematoxylin\u2013eosin staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results on in\u00a0situ expression of inflammasome elements and programmed cell death showed increased expression of IL\u20101\u03b2, NLRP\u20101, caspase\u20101 and RIPK\u20103. We suggest that inflammasome complex participate in the immunopathogenesis in paracoccidioidomycosis oral lesions in an interplay with RIPK3.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13662","type":"journal-article","created":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T05:51:55Z","timestamp":1697262715000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Contribution to the study of inflammasome and programmed cell death in paracoccidioidomycosis oral lesions"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6210-6917","authenticated-orcid":false,"given":"Carla","family":"Pagliari","sequence":"first","affiliation":[{"name":"Departamento de Patologia Faculdade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8097-247X","authenticated-orcid":false,"given":"Luciane","family":"Kanashiro\u2010Galo","sequence":"additional","affiliation":[{"name":"Departamento de Patologia Faculdade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6380-7192","authenticated-orcid":false,"given":"Mirian Nacagami","family":"Sotto","sequence":"additional","affiliation":[{"name":"Departamento de Patologia Faculdade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo 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