{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:16:26Z","timestamp":1787015786577,"version":"build-2736575974"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T00:00:00Z","timestamp":1705536000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Fluconazole\u2010resistant\n                      <jats:italic>Candida parapsilosis<\/jats:italic>\n                      (FRCP) is a matter of concern in Spain.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>\n                      Objective\n                      <jats:bold>s<\/jats:bold>\n                    <\/jats:title>\n                    <jats:p>We here report a FRCP spread across a 777\u2010bed referral hospital located in Burgos, Spain, during the COVID\u201019 pandemic.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients\/Methods<\/jats:title>\n                    <jats:p>\n                      In April 2021, an FRCP isolate (MIC\u2009=\u200964\u2009mg\/L, E\u2010test\u00ae) from a hospitalised patient was detected. Up to June 2022, all\n                      <jats:italic>C.\u2009parapsilosis<\/jats:italic>\n                      isolates (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200935) from hospitalised patients (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200932) were stored and genotyped using microsatellite markers, and their antifungal susceptibilities were studied (EUCAST); FRCP isolates were molecularly characterised.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We detected 26 FRCP isolates collected between 2021 (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098) and 2022 (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200918); isolates were susceptible to amphotericin B, echinocandins and ibrexafungerp. FRCP isolates were grouped into three genotypes: CP\u2010707 and CP\u2010708 involved isolates harbouring the Y132F\u2009+\u2009R398I ERG11p substitutions (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200924) and were clonally related; the remaining CP\u2010675 genotype involved isolates harbouring the G458S ERG11p substitution (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20092). FRCP genotypes were genetically related to the FRCP genotypes found in Madrid and were unrelated to fluconazole\u2010susceptible ones. Patients harbouring FRCP were mainly (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200922\/23) admitted to intensive care units. Most patients had received broad\u2010spectrum antibiotics (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200922\/23), and\/or antifungal therapy with azoles (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200914\/23) within the 30\u2009days prior to FRCP isolation. Thirteen patients were colonised, 10 of whom were infected and presented candidaemia (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098\/10), endovascular infection (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20091\/10) or complicated urinary infection (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20091\/10). Overall nonattributable\u00a030\u2010day mortality was 17% (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20094\/23).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We report an outbreak caused by FRCP affecting patients admitted to the ICU of a referral hospital located in Burgos. Patients harbouring FRCP had a higher fluconazole use than those carrying susceptible isolates.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.13685","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T03:37:05Z","timestamp":1705549025000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Clonal spread of fluconazole\u2010resistant\n                    <i>C. parapsilosis<\/i>\n                    in patients admitted to a referral hospital located in Burgos, Spain, during the COVID\u201019 pandemic"],"prefix":"10.1111","volume":"67","author":[{"given":"Mar\u00eda de los \u00c1ngeles","family":"Mantec\u00f3n\u2010Vallejo","sequence":"first","affiliation":[{"name":"Clinical Microbiology Department Complejo Asistencial Universitario de Burgos  Burgos Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aina","family":"Mesquida","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases Hospital General Universitario Gregorio Mara\u00f1\u00f3n  Madrid Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda de Valle","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Intensive Care Unit Complejo Asistencial Universitario de Burgos  Burgos Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Buz\u00f3n\u2010Mart\u00edn","sequence":"additional","affiliation":[{"name":"Internal Medicine Department Complejo Asistencial Universitario de Burgos  Burgos Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Ossa\u2010Echeverri","sequence":"additional","affiliation":[{"name":"Intensive Care Unit Complejo Asistencial Universitario de Burgos  Burgos Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes","family":"Fisac\u2010Cuadrado","sequence":"additional","affiliation":[{"name":"Intensive Care Unit Complejo Asistencial Universitario de Burgos  Burgos Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregoria","family":"Meg\u00edas\u2010Lob\u00f3n","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Department Complejo Asistencial Universitario de Burgos  Burgos Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Pilar","family":"Ortega\u2010Lafont","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Department Complejo Asistencial Universitario de Burgos  Burgos Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases Hospital General Universitario Gregorio Mara\u00f1\u00f3n  Madrid Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n  Madrid Spain"},{"name":"CIBER Enfermedades Respiratorias\u2010CIBERES (CB06\/06\/0058)  Madrid Spain"},{"name":"Medicine Department, Faculty of Medicine Universidad Complutense de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6254-4570","authenticated-orcid":false,"given":"Pilar","family":"Escribano","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases Hospital General Universitario Gregorio Mara\u00f1\u00f3n  Madrid Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n  Madrid Spain"},{"name":"School of Health, HM Hospitals Universidad Camilo Jos\u00e9 Cela  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7901-8355","authenticated-orcid":false,"given":"Jes\u00fas","family":"Guinea","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases Hospital General Universitario Gregorio Mara\u00f1\u00f3n  Madrid Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n  Madrid Spain"},{"name":"CIBER Enfermedades Respiratorias\u2010CIBERES (CB06\/06\/0058)  Madrid Spain"},{"name":"School of Health, HM Hospitals Universidad Camilo Jos\u00e9 Cela  Madrid 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Open forum","volume":"9","author":"Trevijano\u2010Contador N","year":"2022","journal-title":"Infect Dis"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkac288"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof8111228"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00710-22"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.09.030"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.07.023"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00166"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00986-23"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.13685","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T03:52:15Z","timestamp":1707277935000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.13685"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1111\/myc.13685"],"URL":"https:\/\/doi.org\/10.1111\/myc.13685","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"2023-10-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13685"}}