{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T03:57:39Z","timestamp":1773115059112,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,18]],"date-time":"2024-02-18T00:00:00Z","timestamp":1708214400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Aspergillus fumigatus<\/jats:italic>\u2010specific IgG estimation is crucial for diagnosing allergic bronchopulmonary aspergillosis (ABPA). A point\u2010of\u2010care LDBio immunochromatographic lateral flow assay (LFA) had 0%\u201390% sensitivity to detect IgG\/IgM antibodies against <jats:italic>A. fumigatus<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess the accuracy of LDBio\u2010LFA in diagnosing ABPA, using the modified ISHAM\u2010ABPA working group criteria as the reference standard. The secondary objective was to compare the diagnostic performance between LDBio\u2010LFA and <jats:italic>A. fumigatus<\/jats:italic>\u2010specific IgG (cut\u2010offs, 27 and 40\u2009mgA\/L), using a multidisciplinary team (blinded to <jats:italic>A. fumigatus<\/jats:italic>\u2010IgG and LDBio\u2010LFA results) diagnosis of ABPA as the reference standard.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We prospectively enrolled adult subjects with asthma and ABPA. We performed the LDBio\u2010LFA per the manufacturer's recommendations. We used the commercially available automated fluorescent enzyme immunoassay for measuring serum <jats:italic>A. fumigatus<\/jats:italic>\u2010specific IgG. We used the same serum sample to perform both index tests. The tests were performed by technicians blinded to the results of other tests and clinical diagnoses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We included 123 asthmatic and 166 ABPA subjects, with a mean\u2009\u00b1\u2009SD age of 37.4\u2009\u00b1\u200914.4\u2009years. Bronchiectasis and high\u2010attenuation mucus were seen in 93.6% (146\/156) and 24.3% (38\/156) of the ABPA subjects. The sensitivity and specificity of LDBio\u2010LFA in diagnosing ABPA were 84.9% and 82.9%, respectively. The sensitivity of serum <jats:italic>A. fumigatus<\/jats:italic>\u2010specific IgG \u226527\u2009mgA\/L was 13% better than LDBio\u2010LFA, with no difference in specificity. There was no significant difference in sensitivity and specificity between LDBio\u2010LFA and serum <jats:italic>A. fumigatus<\/jats:italic>\u2010IgG \u226540\u2009mgA\/L.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>LDBio\u2010LFA is a valuable test for diagnosing ABPA. However, a negative test should be confirmed using an enzyme immunoassay.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13700","type":"journal-article","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T03:25:58Z","timestamp":1708313158000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Sensitivity and specificity of <scp>LDBio<\/scp><i>Aspergillus<\/i><scp>ICT<\/scp> lateral flow assay for diagnosing allergic bronchopulmonary aspergillosis in adult asthmatics"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6505-6019","authenticated-orcid":false,"given":"Inderpaul Singh","family":"Sehgal","sequence":"first","affiliation":[{"name":"Department of Pulmonary Medicine Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0410-8468","authenticated-orcid":false,"given":"Valliappan","family":"Muthu","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahajal","family":"Dhooria","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuruswamy Thurai","family":"Prasad","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9097-9253","authenticated-orcid":false,"given":"Shivaprakash M.","family":"Rudramurthy","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashutosh Nath","family":"Aggarwal","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4714-6299","authenticated-orcid":false,"given":"Mandeep","family":"Garg","sequence":"additional","affiliation":[{"name":"Department of Radiodiagnosis Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Pierre","family":"Gangneux","sequence":"additional","affiliation":[{"name":"CHU Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail), UMR_S 1085, Univ Rennes  Rennes France"},{"name":"Laboratoire de Parasitologie\u2010Mycologie, ECMM Excellence Center in Medical Mycology, French National Reference Center on Mycoses and Antifungals (CNRMA LA\u2010Asp C) Centre Hospitalier Universitaire de Rennes  Rennes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arunaloke","family":"Chakrabarti","sequence":"additional","affiliation":[{"name":"Doodhadhari Burfani Hospital  Haridwar Uttarakhand India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2547-7668","authenticated-orcid":false,"given":"Ritesh","family":"Agarwal","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2023.04.009"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.14319"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/lungindia.lungindia_69_23"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.14299"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12141"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12541"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2021.03.023"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13488"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2020.02.012"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12133"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alit.2020.07.006"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00012"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0238855"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmmb.2022.03.002"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2022.11.031"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0252553"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2016-012799"},{"key":"e_1_2_9_19_1","unstructured":"Global Initiative for Asthma (GINA).Global strategy for asthma management and prevention.2023. Accessed 14 November 2023https:\/\/ginasthma.org\/wp\u2010content\/uploads\/2023\/07\/GINA\u20102023\u2010Full\u2010report\u201023_07_06\u2010WMS.pdf"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.130.2.442"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2020.08.043"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.861866"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971-3026.90680"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.4329\/wjr.v4.i4.141"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12874-020-00988-y"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.2307\/2531595"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0295-3"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.13700","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T06:33:30Z","timestamp":1709102010000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.13700"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1111\/myc.13700"],"URL":"https:\/\/doi.org\/10.1111\/myc.13700","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"2023-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13700"}}