{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:29:05Z","timestamp":1789172945949,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T00:00:00Z","timestamp":1711670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Dermatomycoses count to the most frequent dermatoses in Cambodia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this survey was to investigate the occurrence of dermatophytes in this Southeast Asian country.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>From June 2017 to July 2018, skin scrapings were taken from 67 patients with superficial dermatophytosis for mycological diagnostics. Identification of dermatophytes was confirmed by sequencing of the \u2018internal transcribed spacer\u2019\u2014(ITS) region of the rDNA, and the gene of the <jats:italic>Translation Elongation Factor<\/jats:italic> (<jats:italic>TEF<\/jats:italic>)\u2010<jats:italic>1\u03b1<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Patients were suffering from tinea corporis and tinea inguinalis\/cruris 42\/67 (63%), tinea capitis\/faciei 14\/67 (21%), tinea corporis\/capitis\/faciei 6\/67 (9%), tinea manuum\/pedis 2\/67 (3%), tinea pedis 2\/67 (3%) and tinea manuum 1\/67 (1%). Both, by culture and\/or PCR, a dermatophyte was detected in 52 (78%) out of 67 samples. Culture positive were 42 (81%) of 52, PCR positive were 50 (96%). The following dermatophytes were found: <jats:italic>Trichophyton<\/jats:italic> (<jats:italic>T.<\/jats:italic>) <jats:italic>rubrum<\/jats:italic>, 36\/52 strains (69%, 29 by culture), <jats:italic>T<\/jats:italic>.\u2009<jats:italic>mentagrophytes<\/jats:italic>\/<jats:italic>T<\/jats:italic>.\u2009<jats:italic>interdigitale<\/jats:italic> (TM\/TI) 9\/52 (17%, six by culture) and <jats:italic>Microsporum (M.) canis<\/jats:italic> 5\/52 strains (10%, by culture). One strain of <jats:italic>Nannizzia<\/jats:italic> (<jats:italic>N.<\/jats:italic>) <jats:italic>incurvata<\/jats:italic> 1\/52 (2%) and <jats:italic>N.\u2009nana<\/jats:italic> 1\/52 (2%) was isolated. Based on sequencing, we demonstrated that two <jats:italic>T<\/jats:italic>.\u2009<jats:italic>mentagrophytes<\/jats:italic> strains out of the nine TM\/TI represented the new ITS genotype XXV Cambodia. We found one <jats:italic>T<\/jats:italic>.\u2009<jats:italic>mentagrophytes<\/jats:italic> strain genotype VIII (now, reclassified as <jats:italic>T<\/jats:italic>.\u2009<jats:italic>indotineae<\/jats:italic>). This isolate was terbinafine resistant, and it exhibited the amino acid substitution Phe397Leu in the squalene epoxidase. Three strains of <jats:italic>T<\/jats:italic>.\u2009<jats:italic>interdigitale<\/jats:italic> genotype II* were isolated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This is the first survey on epidemiology of dermatophytes in Cambodia. Currently, <jats:italic>T<\/jats:italic>.\u2009<jats:italic>rubrum<\/jats:italic> represents the most frequent species in Cambodia. One Indian strain genotype VIII <jats:italic>T<\/jats:italic>.\u2009<jats:italic>mentagrophytes<\/jats:italic> was found. A highlight was the first description of the new <jats:italic>T<\/jats:italic>.\u2009<jats:italic>mentagrophytes<\/jats:italic> genotype XXV Cambodia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13718","type":"journal-article","created":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T09:34:02Z","timestamp":1711704842000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Dermatophytes and skin dermatophytoses in Southeast Asia\u2014First epidemiological survey from Cambodia"],"prefix":"10.1111","volume":"67","author":[{"given":"Silke","family":"Uhrla\u00df","sequence":"first","affiliation":[{"name":"labopart \u2010 Medizinische Laboratorien  R\u00f6tha OT M\u00f6lbis Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sithach","family":"Mey","sequence":"additional","affiliation":[{"name":"Department of Dermatology Preah Kossamak Hospital  Phnom Penh Cambodia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Koch","sequence":"additional","affiliation":[{"name":"labopart \u2010 Medizinische Laboratorien  R\u00f6tha OT M\u00f6lbis Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"M\u00fctze","sequence":"additional","affiliation":[{"name":"labopart \u2010 Medizinische Laboratorien  R\u00f6tha OT M\u00f6lbis Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constanze","family":"Kr\u00fcger","sequence":"additional","affiliation":[{"name":"labopart \u2010 Medizinische Laboratorien  R\u00f6tha OT M\u00f6lbis Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0695-4003","authenticated-orcid":false,"given":"Michel","family":"Monod","sequence":"additional","affiliation":[{"name":"Dermatology Service Centre Hospitalier Universitaire Vaudois  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Nenoff","sequence":"additional","affiliation":[{"name":"labopart \u2010 Medizinische Laboratorien  R\u00f6tha OT M\u00f6lbis Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,29]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12895-018-0079-8"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/02681219680000471"},{"key":"e_1_2_12_4_1","first-page":"646","article-title":"Mycose osseuse par mycetome \u00e0 grains noirs au Cambodge [Osseous mycosis caused by mycetoma with black granules in Cambodia]","volume":"25","author":"Tournier Lasserve C","year":"1965","journal-title":"Med Trop (Mars)"},{"key":"e_1_2_12_5_1","first-page":"108","article-title":"Myc\u00e9tome fongique a grains noirs, aff. 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