{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T13:59:29Z","timestamp":1793714369825,"version":"4.6.1"},"reference-count":60,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T00:00:00Z","timestamp":1711670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Surveillance studies are crucial for updating trends in\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      species and antifungal susceptibility information.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Determine the\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      species distribution and azole resistance prevalence during this 3\u2010year prospective surveillance study in a Spanish hospital.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Three hundred thirty\u2010five\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      spp. clinical and environmental isolates were collected during a 3\u2010year study. All isolates were screened for azole resistance using an agar\u2010based screening method and resistance was confirmed by EUCAST antifungal susceptibility testing. The azole resistance mechanism was confirmed by sequencing the\n                      <jats:italic>cyp<\/jats:italic>\n                      51A gene and its promoter. All\n                      <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                      strains were genotyped using TRESPERG analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                      was the predominant species recovered with a total of 174 strains (51.94%). The rest of\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      spp. were less frequent:\n                      <jats:italic>Aspergillus niger<\/jats:italic>\n                      (14.93%),\n                      <jats:italic>Aspergillus terreus<\/jats:italic>\n                      (9.55%),\n                      <jats:italic>Aspergillus flavus<\/jats:italic>\n                      (8.36%),\n                      <jats:italic>Aspergillus nidulans<\/jats:italic>\n                      (5.37%) and\n                      <jats:italic>Aspergillus lentulus<\/jats:italic>\n                      (3.28%), among other\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      species (6.57%). TRESPERG analysis showed 99 different genotypes, with 72.73% of the strains being represented as a single genotype. Some genotypes were common among clinical and environmental\n                      <jats:italic>A.\u2009fumigatus<\/jats:italic>\n                      azole\u2010susceptible strains, even when isolated months apart. We describe the occurrence of two azole\u2010resistant\n                      <jats:italic>A.\u2009fumigatus<\/jats:italic>\n                      strains, one clinical and another environmental, that were genotypically different and did not share genotypes with any of the azole\u2010susceptible strains.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                      strains showed a very diverse population although several genotypes were shared among clinical and environmental strains. The isolation of azole\u2010resistant strains from both settings suggest that an efficient analysis of clinical and environmental sources must be done to detect azole resistance in\n                      <jats:italic>A.\u2009fumigatus.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.13719","type":"journal-article","created":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T04:29:32Z","timestamp":1711686572000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Distribution of\n                    <i>Aspergillus<\/i>\n                    species and prevalence of azole resistance in clinical and environmental samples from a Spanish hospital during a three\u2010year study period"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7545-7510","authenticated-orcid":false,"given":"Jose","family":"Lucio","sequence":"first","affiliation":[{"name":"Mycology Reference Laboratory, National Centre for Microbiology Instituto de Salud Carlos III (ISCIII)  Majadahonda Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Alcazar\u2010Fuoli","sequence":"additional","affiliation":[{"name":"Mycology Reference Laboratory, National Centre for Microbiology Instituto de Salud Carlos III (ISCIII)  Majadahonda Madrid Spain"},{"name":"Center for Biomedical Research in Network in Infectious Diseases (CIBERINFEC\u2010CB21\/13\/00105) Instituto de Salud Carlos III  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horacio","family":"Gil","sequence":"additional","affiliation":[{"name":"Mycology Reference Laboratory, National Centre for Microbiology Instituto de Salud Carlos III 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