{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T04:03:28Z","timestamp":1782187408488,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T00:00:00Z","timestamp":1711670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005278","name":"Universidad de Antioquia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005278","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008793","name":"Universidad del Rosario","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008793","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Recurrent vulvovaginal candidiasis (RVVC) is an important and underestimated fungal infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We aimed to determine the fungicidal and proliferative capacities of neutrophils and peripheral blood mononuclear cells (PBMCs), respectively and the clinical and microbiological characteristics of a cohort of Colombian patients diagnosed with RVVC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A cross\u2010sectional study was conducted. A total of 66 women were included (40 diagnosed with RVVC and 26 healthy women [HW]). Demographic and clinical data were recorded. Vaginal fluid samples were obtained for isolation, identification and antifungal susceptibility testing of <jats:italic>Candida<\/jats:italic> species using selective culture media and the Vitek 2.0\u00ae system. Blood samples were also obtained to evaluate cell subpopulations; furthermore, neutrophils and PBMCs were isolated to determine their fungicidal and proliferative capacities, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The median age was 29 (IQR: 34\u201323) for RVVC and 24 (IQR: 30\u201323) for HW. Only two species of the genus <jats:italic>Candida<\/jats:italic> were identified: <jats:italic>Candida albicans<\/jats:italic> (92.5%) and <jats:italic>Candida lusitaniae<\/jats:italic> (7.5%). Resistance to fluconazole, voriconazole, flucytosine and amphotericin B was observed on six <jats:italic>C.\u2009albicans<\/jats:italic> isolates and one <jats:italic>C.\u2009lusitaniae<\/jats:italic> isolate. Only the family history of vulvovaginal candidiasis was associated with RVVC occurrence. The RVVC group exhibited a significantly higher number of neutrophils but with lower fungicidal activity in comparison to HW; likewise, PBMCs from RVVC patients presented a lower proliferation index when stimulated with <jats:italic>C.\u2009albicans<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Contrary to what has been reported worldwide, in Colombian patients with RVVC, <jats:italic>C.\u2009albicans<\/jats:italic> was the main isolated species without increased antifungal resistance. The diminished fungicidal and proliferative capacities of neutrophils and PBMCs, respectively, could suggest a possible alteration in the innate and adaptive immune responses.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13720","type":"journal-article","created":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T09:43:54Z","timestamp":1711705434000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Patients with recurrent vulvovaginal candidiasis exhibit a decrease in both the fungicidal activity of neutrophils and the proliferation of peripheral blood mononuclear cells"],"prefix":"10.1111","volume":"67","author":[{"given":"Jeiser Marcelo","family":"Consuegra\u2010Asprilla","sequence":"first","affiliation":[{"name":"Basic and Applied Microbiology Research Group (MICROBA), School of Microbiology Universidad de Antioquia  Medellin 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