{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:45:09Z","timestamp":1792100709237,"version":"4.4.0"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T00:00:00Z","timestamp":1711670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2024,4]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n The emergence of the pathogenic yeast\n Candida auris<\/jats:italic>\n is of global concern due to its ability to cause hospital outbreaks and develop resistance against all antifungal drug classes. Based on published data for baker's yeast\n Saccharomyces cerevisiae<\/jats:italic>\n , sphingolipid biosynthesis, which is essential for maintaining membrane fluidity and formation of lipid rafts, could offer a target for additive treatment.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n We analysed the susceptibility of\n C<\/jats:italic>\n .\n auris<\/jats:italic>\n to myriocin, which is an inhibitor of the de novo synthesis of sphingolipids in eukaryotic cells in comparison to other\n Candida<\/jats:italic>\n species. In addition, we combined sublethal concentrations of myriocin with the antifungal drugs amphotericin B and fluconazole in\n E<\/jats:italic>\n \u2010tests. Consequently, the combinatory effects of myriocin and amphotericin B were examined in broth microdilution assays.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Myriocin\u2010mediated inhibition of the sphingolipid biosynthesis affected the growth of\n C<\/jats:italic>\n .\n auris<\/jats:italic>\n . Sublethal myriocin concentrations increased fungal susceptibility to amphotericin B. Isolates which are phenotypically resistant (\u22652\u2009mg\/L) to amphotericin B became susceptible in presence of myriocin. However, addition of myriocin had only limited effects onto the susceptibility of\n C<\/jats:italic>\n .\n auris<\/jats:italic>\n against fluconazole.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Our results show that inhibition of de novo sphingolipid biosynthesis increases the susceptibility of\n C<\/jats:italic>\n .\n auris<\/jats:italic>\n to amphotericin B. This may potentially enhance antifungal treatment options fighting this often resistant yeast pathogen.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/myc.13723","type":"journal-article","created":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T05:49:58Z","timestamp":1711691398000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The sphingolipid inhibitor myriocin increases\n Candida auris<\/i>\n susceptibility to amphotericin B"],"prefix":"10.1111","volume":"67","author":[{"given":"Hanna","family":"Stieber","sequence":"first","affiliation":[{"name":"Institute for Hygiene and Microbiology University of W\u00fcrzburg W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"Junghanns","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Microbiology University of W\u00fcrzburg W\u00fcrzburg 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