{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:57:53Z","timestamp":1791615473094,"version":"4.3.4"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T00:00:00Z","timestamp":1717027200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The prevalence of <jats:italic>Candida glabrata<\/jats:italic> healthcare\u2010associated infections is on the rise worldwide and in Lebanon, <jats:italic>Candida glabrata<\/jats:italic> infections are difficult to treat as a result of their resistance to azole antifungals and their ability to form biofilms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The first objective of this study was to quantify biofilm biomass in the most virulent <jats:italic>C.\u2009glabrata<\/jats:italic> isolates detected in a Lebanese hospital. In addition, other pathogenicity attributes were evaluated. The second objective was to identify the mechanisms of azole resistance in those isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A mouse model of disseminated systemic infection was developed to evaluate the degree of virulence of 41 azole\u2010resistant <jats:italic>C.\u2009glabrata<\/jats:italic> collected from a Lebanese hospital. The most virulent isolates were further evaluated alongside an isolate having attenuated virulence and a reference strain for comparative purposes. A DNA\u2010sequencing approach was adopted to detect single nucleotide polymorphisms (SNPs) leading to amino acid changes in proteins involved in azole resistance and biofilm formation. This genomic approach was supported by several phenotypic assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All chosen virulent isolates exhibited increased adhesion and biofilm biomass compared to the isolate having attenuated virulence. The amino acid substitutions D679E and I739N detected in the subtelomeric silencer Sir3 are potentially involved\u2014 in increased adhesion. In all isolates, amino acid substitutions were detected in the ATP\u2010binding cassette transporters Cdr1 and Pdh1 and their transcriptional regulator Pdr1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In summary, increased adhesion led to stable biofilm formation since mutated Sir3 could de\u2010repress adhesins, while decreased azole susceptibility could result from mutations in Cdr1, Pdh1 and Pdr1.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13750","type":"journal-article","created":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T12:15:49Z","timestamp":1717071349000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Adhesive and biofilm\u2010forming <i>Candida glabrata<\/i> Lebanese hospital isolates harbour mutations in subtelomeric silencers and 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