{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:19:07Z","timestamp":1767853147038,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T00:00:00Z","timestamp":1718582400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Serum galactomannan (GM) testing is essential for diagnosing invasive aspergillosis (IA), particularly in immunocompromised individuals. The global lack of on\u2010site GM testing capacities necessitates cost\u2010effective alternatives, such as .the clarus <jats:italic>Aspergillus<\/jats:italic> GM enzyme immunoassay prototype (clarus AGM prototype).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This single\u2010centre, cross\u2010sectional study compared the diagnostic performance of the clarus AGM prototype (IMMY, Norman, Oklahoma) with the serological gold standard (=Platelia AGM assay; Bio\u2010Rad, Marnes\u2010la\u2010Cocquette, France). IA was classified according to modified 2020 EORTC\/MSG consensus and 2024 FUNDICU criteria. In total, 300 prospectively (May\u2010Dec 2023) and retrospectively (2012\u20132015) collected samples were included.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among 300 samples from 232 patients, 49 (16%) were classified as proven (<jats:italic>n<\/jats:italic>\u2009=\u20091) or probable IA (<jats:italic>n<\/jats:italic>\u2009=\u200948). In non\u2010IA cases (<jats:italic>n<\/jats:italic>\u2009=\u2009250), one patient was classified as possible IA. With the manufacturer recommended cut\u2010off of \u22650.2, sensitivity and specificity of the clarus AGM prototype were 27% (13\/49; 95% confidence interval [CI]: 15%\u201341%) and 99% (248\/250; 95% CI: 97%\u2013100%), respectively, while sensitivity and specificity were 78% and 79% when using the optimised Youden's cut\u2010off of 0.0045 ODI. ROC curve analysis demonstrated an area under the curve (AUC) of 0.829 (95% CI: 0.760\u20130.898) for the clarus AGM prototype in distinguishing between proven\/probable IA and non\u2010IA. The AUC for the Platelia AGM was 0.951 (95% CI: 0.909\u2013994). Spearman's correlation analysis showed a weak correlation between the two assays (0.382; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The weak correlation between the clarus AGM prototype and Platelia AGM highlights the need for further investigation into the clinical performance of the clarus AGM prototype, giving the different antigen epitopes addressed.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13756","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T02:18:56Z","timestamp":1718677136000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Performance of the clarus <i>Aspergillus<\/i> galactomannan enzyme immunoassay prototype for the diagnosis of invasive pulmonary aspergillosis in serum"],"prefix":"10.1111","volume":"67","author":[{"given":"Johannes","family":"Boyer","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine Medical University of Graz, ECMM Excellence Center  Graz Austria"},{"name":"Translational Mycology Medical University of Graz  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3070-2508","authenticated-orcid":false,"given":"Sarah","family":"Sedik","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine Medical University of Graz, ECMM Excellence Center  Graz Austria"},{"name":"Translational Mycology Medical University of Graz  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Egger","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine Medical University of Graz, ECMM Excellence Center  Graz Austria"},{"name":"Translational Mycology Medical University of Graz  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl","family":"Dichtl","sequence":"additional","affiliation":[{"name":"Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5751-9311","authenticated-orcid":false,"given":"Juergen","family":"Prattes","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine Medical University of Graz, ECMM Excellence Center  Graz Austria"},{"name":"Translational Mycology Medical University of Graz  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Kriegl","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine Medical University of Graz, ECMM Excellence Center  Graz Austria"},{"name":"Translational Mycology Medical University of Graz  Graz Austria"},{"name":"BioTechMed\u2010Graz  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Krause","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine Medical University of Graz, ECMM Excellence Center  Graz Austria"},{"name":"Translational Mycology Medical University of Graz  Graz Austria"},{"name":"BioTechMed\u2010Graz  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian","family":"Pr\u00fcller","sequence":"additional","affiliation":[{"name":"Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1653-2824","authenticated-orcid":false,"given":"Martin","family":"Hoenigl","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine Medical University of Graz, ECMM Excellence Center  Graz Austria"},{"name":"Translational Mycology Medical University of Graz  Graz Austria"},{"name":"BioTechMed\u2010Graz  Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,6,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof3040057"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mmcr.2018.10.006"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2014-206291"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.08.014"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-021-06510-2:1-2"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-022-01172-2"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-86-5-539"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-024-00831-8"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw326"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2018.01.002"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz1008"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-024-07341-7"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(22)00261-0"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2023.106718:106718"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00068-08"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01689-21:Jcm0168921"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1281"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-023-00739-9"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myz079"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2023.2257597"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01229-21:Jcm0122921"},{"key":"e_1_2_9_23_1","article-title":"Performance of the clarus aspergillus galactomannan enzyme immunoassay prototype for the diagnosis of invasive pulmonary aspergillosis in serum","author":"Sedik S Boyer J","year":"2024","journal-title":"Mycopathologia"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-04-351601"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.02.005"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13352"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2020.1820864:1-9"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03500-13"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myw102"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2018.05.001"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01476\u201023"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.13756","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T05:33:45Z","timestamp":1721626425000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.13756"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1111\/myc.13756"],"URL":"https:\/\/doi.org\/10.1111\/myc.13756","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"2024-05-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13756"}}