{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T12:22:04Z","timestamp":1789474924541,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T00:00:00Z","timestamp":1721779200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Candida vulturna<\/jats:italic> is an emerging pathogen belonging to the <jats:italic>Metshnikowiaceae<\/jats:italic> family together with <jats:italic>Candida auris<\/jats:italic> and <jats:italic>Candida haemulonii<\/jats:italic> species complex. Some strains of this species were reported to be resistant to several antifungal agents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study aims to address identification difficulties, evaluate antiungal susceptibilities and explore the molecular mechanisms of azole resistance of <jats:italic>Candida vulturna<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We studied five <jats:italic>C<\/jats:italic>. <jats:italic>vulturna<\/jats:italic> clinical strains isolated in three Colombian cities. Identification was performed by phenotypical, proteomic and molecular methods. Antifungal susceptibility testing was performed following CLSI protocol. Its <jats:italic>ERG11<\/jats:italic> genes were sequenced and a substitution was encountered in azole resistant isolates. To confirm the role of this substitution in the resistance phenotype, <jats:italic>Saccharomyces cerevisiae<\/jats:italic> strains with a chimeric <jats:italic>ERG11<\/jats:italic> gene were created.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Discrepancies in identification methods are highlighted. Sequencing confirmed the identification as <jats:italic>C<\/jats:italic>. <jats:italic>vulturna<\/jats:italic>. Antifungal susceptibility varied among strains, with four strains exhibiting reduced susceptibility to azoles and amphotericin B. <jats:italic>ERG11<\/jats:italic> sequencing showed a point mutation (producing a P135S substitution) that was associated with the azole\u2010resistant phenotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study contributes to the understanding of <jats:italic>C<\/jats:italic>. <jats:italic>vulturna<\/jats:italic>'s identification challenges, its susceptibility patterns, and sheds light on its molecular mechanisms of azole resistance.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13757","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T04:15:40Z","timestamp":1721880940000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Mechanism of azole resistance in <i>Candida vulturna<\/i>, an emerging multidrug resistant pathogen related with <i>Candida haeumulonii<\/i> and <i>Candida auris<\/i>"],"prefix":"10.1111","volume":"67","author":[{"given":"Daiana","family":"Macedo","sequence":"first","affiliation":[{"name":"Facultad de Bioqu\u00edmica Universidad Nacional del Litoral  Santa Fe Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Santa Fe Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indira","family":"Berrio","sequence":"additional","affiliation":[{"name":"Corporaci\u00f3n para Investigaciones Biol\u00f3gicas  Medell\u00edn Colombia"},{"name":"Hospital General de Medell\u00edn Luz Castro Guti\u00e9rrez ESE  Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Escandon","sequence":"additional","affiliation":[{"name":"Grupo de Microbiologia Instituto Nacional de Salud  Bogot\u00e1 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