{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:52:34Z","timestamp":1793145154303,"version":"4.5.0"},"reference-count":20,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T00:00:00Z","timestamp":1722988800000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Rapid galactomannan tests, such as the s\u00f5na <jats:italic>Aspergillus<\/jats:italic> GM Lateral Flow Assay (GM\u2010LFA) and the <jats:italic>Aspergillus<\/jats:italic> Galactomannan Ag VIRCLIA\u00ae Monotest (GM\u2010Monotest), which are suitable for the analysis of single samples, have the potential to accelerate diagnosis of invasive aspergillosis (IA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To compare the performance of the GM\u2010Monotest and the GM\u2010LFA for the diagnosis of IA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients\/Methods<\/jats:title><jats:p>Two patient cohorts were analysed: adults who had received an allogeneic haematopoietic stem\u2010cell transplant (alloHSCT\u2010cohort) and patients with proven\/probable IA from a 5\u2010year period (cross\u2010sectional IA\u2010cohort). In the alloHSCT\u2010cohort, weekly serum samples were tested, whereas in the cross\u2010sectional IA\u2010cohort sera and bronchoalveolar lavage fluids were analysed. The diagnostic performance was calculated using two definitions for positivity: (1) a single positive GM result and (2) at least two positive GM results from consecutive samples. IA classification followed EORTC\/MSG 2019.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The alloHSCT\u2010cohort included 101 patients. Four had proven\/probable IA, 26 possible IA and 71 no IA. The specificity for one positive serum and two consecutively positive sera was 88.7% and 100% (GM\u2010Monotest) and 85.9% and 98.6% (GM\u2010LFA). Comparison of ROC curves in the alloHSCT\u2010cohort showed no significant difference. The cross\u2010sectional IA\u2010cohort included 59 patients with proven\/probable IA. The sensitivity for one positive sample and two consecutively positive samples was 83.1% and 55.1% (GM\u2010Monotest) and 86.4% and 71.4% (GM\u2010LFA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Both assays showed comparable diagnostic performance with a higher sensitivity for the GM\u2010LFA if two consecutive positive samples were required for positivity. However, due to poor reproducibility, positive GM\u2010LFA results should always be confirmed.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13782","type":"journal-article","created":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T06:48:49Z","timestamp":1723013329000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The <i>Aspergillus<\/i> galactomannan Ag <scp>VIRCLIA<\/scp>\u00ae Monotest and the s\u00f5na <i>Aspergillus<\/i> galactomannan lateral flow assay show comparable performance for the diagnosis of invasive aspergillosis"],"prefix":"10.1111","volume":"67","author":[{"given":"Corinna","family":"K\u00fcpper","sequence":"first","affiliation":[{"name":"Mikrobiologisches Institut\u2014Klinische Mikrobiologie, Immunologie und Hygiene, Universit\u00e4tsklinikum Erlangen und Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy Moritz","family":"Erb","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut\u2014Klinische Mikrobiologie, Immunologie und Hygiene, Universit\u00e4tsklinikum Erlangen und Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Tr\u00e4ger","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut\u2014Klinische Mikrobiologie, Immunologie und Hygiene, Universit\u00e4tsklinikum Erlangen und Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Meintker","sequence":"additional","affiliation":[{"name":"Department f\u00fcr H\u00e4matologie\/Onkologie, Medizinische Klinik 5 Universit\u00e4tsklinikum Erlangen und Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Valenza","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut\u2014Klinische Mikrobiologie, Immunologie und Hygiene, Universit\u00e4tsklinikum Erlangen und Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Bogdan","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut\u2014Klinische Mikrobiologie, Immunologie und Hygiene, Universit\u00e4tsklinikum Erlangen und Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen Germany"},{"name":"FAU Profile Center for Immunomedicine, FAU Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1130-9727","authenticated-orcid":false,"given":"J\u00fcrgen","family":"Held","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut\u2014Klinische Mikrobiologie, Immunologie und Hygiene, Universit\u00e4tsklinikum Erlangen und Friedrich\u2010Alexander\u2010Universit\u00e4t (FAU) Erlangen\u2010N\u00fcrnberg  Erlangen 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