{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:29:54Z","timestamp":1790720994211,"version":"4.1.0"},"reference-count":89,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T00:00:00Z","timestamp":1724112000000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006182","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e Inova\u00e7\u00e3o do Esp\u00edrito Santo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006182","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p><jats:italic>Candida<\/jats:italic> spp. is an opportunistic pathogen that causes superficial and invasive infections with nosocomial outbreaks without strict hygiene protocols. Herein, we assessed oral colonisation by <jats:italic>Candida<\/jats:italic> spp. in 209 Intensive Care Unit (ICU) patients between July 2021 and April 2022, conducting clinical, epidemiological, and microbiological characterisation of those developing oral or invasive candidiasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Initial oral swabs were collected within 24\u2009h of admission in the ICU, followed by collections on Days 2, 4, 6 and 8. Swabs from denture\u2010wearing patients, abiotic surfaces, healthcare professionals' hands, and retroauricular regions were also obtained. Recovered yeasts and filamentous fungi were identified using MALDI\u2010TOF MS and morphological characteristics, respectively. Genetic similarity of <jats:italic>Candida<\/jats:italic> spp. isolates was evaluated using Amplified fragment length polymorphism (AFLP), and the antifungal susceptibility profile was determined by broth microdilution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the study, 64.11% of patients were orally colonised by <jats:italic>Candida<\/jats:italic> spp. Of these, 80.59% were colonised within the first 24\u2009h. Oral colonisation also occurred on subsequent days: 50%\/Day 2, 26.92%\/Day 4, and 11.53%\/Days 6 and 8. Of the patients, 8.61% had oral candidiasis, mainly pseudomembranous. Among orally colonised patients, 2.23% developed invasive candidiasis. Besides, 89.47% of healthcare professionals evaluated were colonised. MALDI\u2010TOF MS identified different yeast species, and <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> (45.34%), <jats:italic>C<\/jats:italic>. <jats:italic>tropicalis<\/jats:italic> (15.7%), and <jats:italic>C<\/jats:italic>. <jats:italic>parapsilosis<\/jats:italic> sensu stricto (9.88%) were the most prevalent. AFLP analysis indicated a high genetic correlation (\u226597%) between <jats:italic>C<\/jats:italic>. <jats:italic>parapsilosis<\/jats:italic> sensu stricto isolates from patients and professionals. Three resistant <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> isolates were also found.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study reported a diversity of yeast and filamentous fungi species in ICU patients and highlighted early <jats:italic>Candida<\/jats:italic> spp. colonisation risks for invasive candidiasis, as well as the potential horizontal transmission in the nosocomial setting, emphasising the need for effective infection control measures.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13786","type":"journal-article","created":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T05:05:56Z","timestamp":1724130356000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Early <i>Candida<\/i> colonisation impact on patients and healthcare professionals in an intensive care unit"],"prefix":"10.1111","volume":"67","author":[{"given":"Yago R.","family":"Dalben","sequence":"first","affiliation":[{"name":"Center for Research in Medical Mycology (CIMM), Health Sciences Center (CCS) Federal University of Esp\u00edrito Santo (UFES)  Vit\u00f3ria Esp\u00edrito Santo Brazil"},{"name":"Infectious Diseases Postgraduate Program, Health Sciences Center (CCS) Federal University of Esp\u00edrito Santo (UFES)  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5121-2076","authenticated-orcid":false,"given":"Jhully","family":"Pimentel","sequence":"additional","affiliation":[{"name":"Center for Research in Medical Mycology (CIMM), Health Sciences Center (CCS) Federal University of Esp\u00edrito Santo (UFES)  Vit\u00f3ria Esp\u00edrito Santo Brazil"},{"name":"Infectious Diseases Postgraduate Program, Health Sciences Center (CCS) Federal University of Esp\u00edrito Santo (UFES)  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone B.","family":"Maifrede","sequence":"additional","affiliation":[{"name":"Pathology Department, Health Sciences Center (CCS) Federal University of Esp\u00edrito Santo (UFES)  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamile A.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Parasitology, Discipline of Cellular Biology, Laboratory of Emerging Fungal Pathogens Federal University of S\u00e3o Paulo (UNIFESP)  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco O.","family":"Bessa\u2010Neto","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Antimicrobial Resistance (LEARN), Biological Sciences Department (DCB) Institute of Environmental, Chemical, and Pharmaceutical Sciences (ICAQF), Federal University of S\u00e3o Paulo (UNIFESP)  Diadema S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Fabr\u00edcio S.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Interdisciplinary Care Department Cassiano Ant\u00f4nio Moraes University Hospital (HUCAM), Health Sciences Center (CCS), Federal University of Esp\u00edrito Santo (UFES)  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo R.","family":"Leite","sequence":"additional","affiliation":[{"name":"Pathology Department, Health Sciences Center (CCS) Federal University of Esp\u00edrito Santo (UFES)  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0891-4563","authenticated-orcid":false,"given":"Anderson M.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Parasitology, Discipline of Cellular Biology, Laboratory of Emerging Fungal Pathogens Federal University of S\u00e3o Paulo (UNIFESP)  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Cay\u00f4","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Antimicrobial Resistance (LEARN), Biological Sciences Department (DCB) Institute of Environmental, Chemical, and Pharmaceutical Sciences (ICAQF), Federal University of S\u00e3o Paulo (UNIFESP)  Diadema S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6865-7955","authenticated-orcid":false,"given":"T\u00e2nia Regina","family":"Gr\u00e3o\u2010Velloso","sequence":"additional","affiliation":[{"name":"Dental Clinic Department, Health Sciences Center (CCS) Federal University of Esp\u00edrito Santo (UFES)  Vit\u00f3ria Esp\u00edrito Santo Brazil"},{"name":"Dental Science Postgraduate Program, Health Sciences Center (CCS) Federal University of Esp\u00edrito Santo (UFES)  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4170-4445","authenticated-orcid":false,"given":"Sarah S.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Center for Research in Medical Mycology (CIMM), Health Sciences Center (CCS) Federal University of Esp\u00edrito Santo (UFES)  Vit\u00f3ria Esp\u00edrito Santo Brazil"},{"name":"Infectious Diseases Postgraduate Program, Health Sciences Center (CCS) Federal University of Esp\u00edrito Santo (UFES)  Vit\u00f3ria Esp\u00edrito Santo Brazil"},{"name":"Pathology Department, Health Sciences Center (CCS) Federal University of Esp\u00edrito Santo (UFES)  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010012"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2020.1820292"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/adj.12335"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4372-x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7020079"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-92300-0_4"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146909"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S378629"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2021.08.081"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42399-022-01148-7"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-1577-0_22"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780601164306"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780410001731574"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/315193"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0197925"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bdj.2017.886"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00808-07"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/23.21.4407"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008330"},{"issue":"3","key":"e_1_2_10_21_1","first-page":"314","article-title":"Construction of a genetic linkage map in man using restriction fragment length polymorphisms","volume":"32","author":"Botstein D","year":"1980","journal-title":"Am J Hum Genet"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00564200"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001220051054"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001220051046"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.29327\/24816.4.3-5"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/1369378031000147458"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2008.02.006"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2012.08.001"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7020077"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.4034\/PBOCI.2012.124.11"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.9771\/cmbio.v19i2.36692"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.13243"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-023-05008-z"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.5935\/0103-507X.20140058"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afw147"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0242832"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-016-2684-y"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0041-1735797"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2004.06.033"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2020.101110"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12685"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.15167\/2421-4248\/JPMH2021.62.1.1645"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.10.044"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjm.2015.05.001"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-020-00710-z"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2006.01348.x"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2022.2117093"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00232"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2022.2153086"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.676177"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myaa098"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof8020183"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof8020100"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep27099"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.15607"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.5604\/17331331.1197283"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9071536"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines10111881"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-007-0316-2"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11061442"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/s100960000348"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-022-04164-0"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1590\/1807-3107bor-2017.vol31.0038"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1590\/1519-6984.169623"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo13040580"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021449"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof5020030"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S262311"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof8030310"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1590\/0074-02760160166"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics13101529"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof6040374"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1001845"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.00554"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.15226\/sojmid\/6\/2\/00194"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268820001624"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01614-08"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01629"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11071685"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2019.04.016"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12112712"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-37855-2"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01177-15"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-016-1767-9"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-022-02824-6"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof6040211"},{"key":"e_1_2_10_87_1","volume-title":"Acute Respiratory Distress Syndrome","author":"Diamond M","year":"2024"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S118892"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1136217"},{"key":"e_1_2_10_90_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8020154"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.13786","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T09:05:56Z","timestamp":1755507956000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.13786"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8]]},"references-count":89,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1111\/myc.13786"],"URL":"https:\/\/doi.org\/10.1111\/myc.13786","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8]]},"assertion":[{"value":"2024-05-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13786"}}