{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T05:10:08Z","timestamp":1751173808011,"version":"3.41.0"},"reference-count":20,"publisher":"Wiley","issue":"7-8","license":[{"start":{"date-parts":[[2017,12,19]],"date-time":"2017-12-19T00:00:00Z","timestamp":1513641600000},"content-version":"vor","delay-in-days":10033,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[1990,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Two<jats:italic>Blastomyces dermatitidis<\/jats:italic>commercial immunodiffusion antigens, Meridian Diagnostics and Nolan\u2010Scott Laboratories, and two<jats:italic>B. dermatitidis<\/jats:italic>yeast phase lysate antigens, T\u201058 and K\u2010Le, prepared in our laboratory were utilized in an enzyme\u2010linked immunosorbent assay (ELISA) for the detection of antibodies in 61 serum specimens from dogs with blastomycosis. All antigens used in the ELISA were diluted to 50 ng of protein ml<jats:sup>\u22121<\/jats:sup>. Immunodiffusion (ID) tests were also performed on the sera using a Nolan\u2010Scott ID antigen. Greater reactivity, based on the ELISA index value, was evidenced with the two yeast phase lysate antigens over the two commercial reagents in the ELISA. In addition the sensitivity of the ELISA with the T\u201058, K\u2010Le and Meridian antigens was considerably greater than that of the ID test. The Nolan\u2010Scott antigen, however, was able to detect more positive specimens when used in the ID procedure than in the ELISA. Therefore encouraging results were obtained with respect to the continued development and optimization of the ELISA using<jats:italic>B. dermatitidis<\/jats:italic>cell lysate antigens for the diagnosis of blastomycosis.<\/jats:p>","DOI":"10.1111\/myc.1990.33.7-8.375","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T08:37:40Z","timestamp":1514018260000},"page":"375-381","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of enzyme immunoassay and immunodiffusion for the detection of canine blastomycosis"],"prefix":"10.1111","volume":"33","author":[{"given":"B. W.","family":"Seawell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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