{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T22:14:51Z","timestamp":1715984091977},"reference-count":6,"publisher":"Wiley","issue":"9-10","license":[{"start":{"date-parts":[[2017,12,20]],"date-time":"2017-12-20T00:00:00Z","timestamp":1513728000000},"content-version":"vor","delay-in-days":8511,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[1994,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>A total of 178 sera, including 68 from proven cases of histoplasmosis (65 positive for the presence of <jats:italic>Histoplasma capsulatum<\/jats:italic> var. <jats:italic>capsulatum<\/jats:italic> antibodies and three positive for antigen), 93 from patients with suspected histoplasmosis but with no laboratory evidence of <jats:italic>H. capsulatum<\/jats:italic> var. <jats:italic>capsulatum<\/jats:italic> infection, 14 from humans with heterologous fungal and non\u2010fungal infections and three from normal individuals, were tested for IgG <jats:italic>H. capsulatum<\/jats:italic> antibodies and M or M and H precipitins by enzyme immunoassay (EIA) (Meridian Diagnostics, Cincinnati, OH, USA) and microimmunodiffusion (MID) respectively. Sixty\u2010three of the 68 histoplasmosis case sera demonstrated IgG antibody, and 65 of 68 demonstrated the presence of specific precipitins in the MID test. Nine positive case sera, when tested with the Laboratory Branch complement fixation (LBCF) test, reacted positively to whole yeast and histoplasmin antigens (titres 1 : 8 to 1 : 512). Three histoplasmosis case sera repeatedly tested negative for IgG, specific precipitins and complement\u2010fixing antibodies, whereas they were positive for <jats:italic>Histoplasma<\/jats:italic> antigen. Eighteen of 95 sera from patients without evidence of histoplasmosis demonstrated IgG antibody in the EIA only. Among these positive sera, three out of three cases of aspergillosis and three out of five cases of blastomycosis were confirmed. Sera from HIV\u2010infected and healthy individuals did not show IgG or M and\/or H antibodies to <jats:italic>H. capsulatum<\/jats:italic>. Ninety\u2010three sera were negative by both EIA and MID. The EIA for IgG was less sensitive (97%) than MID (100%). The specificity of EIA and MID was 84% and 100% respectively.<\/jats:p>","DOI":"10.1111\/myc.1994.37.9-10.313","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T10:54:25Z","timestamp":1514026465000},"page":"313-316","source":"Crossref","is-referenced-by-count":8,"title":["Comparative evaluation of the Premier<sup>\u2122<\/sup> enzyme immunoassay, micro\u2010immunodiffusion and complement fixation tests for the detection of <i>Histoplasma capsulatum<\/i> var. <i>capsulatum<\/i> antibodies"],"prefix":"10.1111","volume":"37","author":[{"given":"A. S.","family":"Sekhon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Kaufman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. S.","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Moledina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Jalbert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. H.","family":"Notenboom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,20]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46651992000600018"},{"key":"e_1_2_1_3_1","first-page":"924","volume-title":"Manual of Clinical Microbiology","author":"Kaufman L.","year":"1985"},{"key":"e_1_2_1_4_1","first-page":"191","volume-title":"Serodiagnosis of Mycotic Diseases, American Lecture Series","author":"Palmer D. F.","year":"1977"},{"key":"e_1_2_1_5_1","first-page":"506","volume-title":"Manual of Clinical Laboratory Immunology","author":"Kaufman L.","year":"1992"},{"key":"e_1_2_1_6_1","volume-title":"Agar Immunodiffusion Tests","author":"Kaufman L.","year":"1972"},{"key":"e_1_2_1_7_1","volume-title":"Premier TMHistoplasma EIA, Package Insert, Catalog","author":"Diagnostic Meridian","year":"1992"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.1994.37.9-10.313","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.1994.37.9-10.313","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T21:11:26Z","timestamp":1696021886000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.1994.37.9-10.313"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,9]]},"references-count":6,"journal-issue":{"issue":"9-10","published-print":{"date-parts":[[1994,9]]}},"alternative-id":["10.1111\/myc.1994.37.9-10.313"],"URL":"https:\/\/doi.org\/10.1111\/myc.1994.37.9-10.313","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,9]]}}}