{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:30:58Z","timestamp":1753878658012,"version":"3.41.2"},"reference-count":23,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T00:00:00Z","timestamp":1732060800000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Previous studies correlated Sensititre YeastOne and gradient diffusion plastic strips with standard procedures for the detection of echinocandin\u2010resistant <jats:styled-content style=\"fixed-case\"><jats:italic>C.\u2009glabrata<\/jats:italic><\/jats:styled-content> isolates. However, these studies were limited by the low number of resistant isolates studied; the inclusion of sufficient numbers of mutant isolates is essential to test the procedures' capacity to detect resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We assessed the performance of Sensititre YeastOne and Etest strips to detect echinocandin resistance in susceptible or resistant <jats:styled-content style=\"fixed-case\"><jats:italic>C.\u2009glabrata<\/jats:italic><\/jats:styled-content> isolates (<jats:italic>n<\/jats:italic>\u2009=\u200980) in which the <jats:italic>FKS<\/jats:italic> genes were sequenced, and MICs interpreted using EUCAST and CLSI breakpoints.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients\/Methods<\/jats:title><jats:p>Isolates were echinocandin\u2010susceptible (<jats:italic>n<\/jats:italic>\u2009=\u200950) or echinocandin\u2010resistant according to EUCAST 7.4 methodology. Echinocandin susceptibility using Sensititre YeastOne and Etest strips and categorical agreement were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All except one anidulafungin\u2010resistant isolate had an anidulafungin Sensititre YeastOne MIC\u2009\u2265\u20090.25\u2009mg\/L, while most micafungin\u2010resistant isolates had a MIC\u2009\u2265\u20090.25\u2009mg\/L. Likewise, all anidulafungin\u2010resistant isolates had an anidulafungin Etest strip\u2010obtained MIC\u2009\u2265\u20090.03\u2009mg\/L, whereas micafungin\u2010resistant isolates were \u2265\u20090.125\u2009mg\/L. Overall, these commercial methods correctly classified &gt;\u200990% of isolates by using any breakpoint. Despite the low number of errors detected, these were mostly false resistance (major errors) with EUCAST breakpoints and false susceptibility (very major errors) with CLSI breakpoints.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Sensititre YeastOne and Etest strips were suitable procedures to detect echinocandin resistance in <jats:styled-content style=\"fixed-case\"><jats:italic>C.\u2009glabrata<\/jats:italic><\/jats:styled-content>. The high number of FKS mutants included reinforces our study and opens the door for multicentre validations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.70003","type":"journal-article","created":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T02:29:18Z","timestamp":1732156158000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detecting Echinocandin Resistance in <scp><i>C.\u2009glabrata<\/i><\/scp> Using Commercial Methods: Are <scp>CLSI<\/scp> or <scp>EUCAST<\/scp> Breakpoints Suitable for Categorical Classification?"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6254-4570","authenticated-orcid":false,"given":"Pilar","family":"Escribano","sequence":"first","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Universidad Complutense de Madrid  Madrid Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n  Madrid Spain"},{"name":"Facultad HM de Ciencias de la Salud Universidad Camilo Jos\u00e9 Cela  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura Alguacil","family":"Cu\u00e9llar","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Universidad Complutense de Madrid  Madrid Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Almudena","family":"\u00c1lvarez\u2010Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Universidad Complutense de Madrid  Madrid Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Universidad Complutense de Madrid  Madrid Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n  Madrid Spain"},{"name":"CIBER Enfermedades Respiratorias\u2010CIBERES (CB06\/06\/0058)  Madrid Spain"},{"name":"Medicine Department, Faculty of Medicine Universidad Complutense de Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"Guinea","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Universidad Complutense de Madrid  Madrid Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n  Madrid Spain"},{"name":"Facultad HM de Ciencias de la Salud Universidad Camilo Jos\u00e9 Cela  Madrid Spain"},{"name":"CIBER Enfermedades Respiratorias\u2010CIBERES (CB06\/06\/0058)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2023.08.021"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00986-23"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000215"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04134-14"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01249-21"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkac288"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01935.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2011.01.004"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01256-09"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02445-09"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00512-17"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01027-16"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00740-20"},{"key":"e_1_2_9_15_1","first-page":"1","article-title":"EUCAST Definitive Document E. Def 7.4: Method for the Determination of Broth Dilution Minimum Inhibitory Concentrations of Antifungal Agents for Yeasts","author":"Guinea J.","year":"2023","journal-title":"European Committee for Antimicrobial Susceptibility Testing (EUCAST)"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02004-18"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00162-18"},{"issue":"1","key":"e_1_2_9_18_1","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.01876-18","article-title":"MSH2 Gene Point Mutations Are Not Antifungal Resistance Markers in Candida glabrata","volume":"63","author":"Bordallo\u2010Cardona M. 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