{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T19:29:39Z","timestamp":1774380579318,"version":"3.50.1"},"reference-count":76,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T00:00:00Z","timestamp":1737590400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011893","name":"Pan American Health Organization","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011893","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Epidemiological surveillance of <jats:italic>Candidozyma<\/jats:italic> sp. has become important because many species of this new genus have been reported to be responsible for nosocomial outbreaks and to exhibit elevated minimal inhibitory concentrations (MIC) to one or more classes of antifungal drugs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To describe the genetic relationships among Argentinian clinical isolates belonging to the <jats:italic>Candidozyma<\/jats:italic> genus and to study the molecular mechanisms associated with antifungal resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed whole\u2010genome sequencing of 41 isolates. Identification was based on ribosomal DNA sequencing and susceptibility testing was determined according to the EUCAST document. Phylogenetic analysis, non\u2010synonymous mutations in genes associated with antifungal resistance and the presence of copy number variations (CNVs) were investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified 12 <jats:italic>Candidozyma haemuli<\/jats:italic>, 11 <jats:italic>Candidozyma haemuli var<\/jats:italic>. <jats:italic>vulneris<\/jats:italic>, 5 <jats:italic>Cz<\/jats:italic>. <jats:italic>haemuli\/ Cz<\/jats:italic>. <jats:italic>haemuli var<\/jats:italic>. <jats:italic>vulneris<\/jats:italic> ITS hybrids, 8 <jats:italic>Candidozyma duobushaemuli<\/jats:italic> and 5 <jats:italic>Candidozyma cf. pseudohaemuli.<\/jats:italic> Phylogenetic analysis, together with clinical data, demonstrated nosocomial transmission events. In addition, <jats:italic>Cz. haemuli<\/jats:italic> and <jats:italic>Cz. haemuli var. vulneris<\/jats:italic> were not separated in the phylogenetic tree; the <jats:italic>Cz. cf. pseudohaemuli<\/jats:italic> isolates clustered distantly from the <jats:italic>Cz. pseudohaemuli<\/jats:italic> type strain. Most isolates were resistant to amphotericin B, and two <jats:italic>Cz. haemuli<\/jats:italic> isolates showed fluconazole resistance and Y132F mutation in <jats:italic>ERG11<\/jats:italic>. We did not find CNV in genes associated with antifungal resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings highlight the need for epidemiological surveillance of these species and the study of molecular mechanisms associated with antifungal resistance. Furthermore, we propose a taxonomic revision for <jats:italic>Cz. haemuli var. vulneris<\/jats:italic> and <jats:italic>Cz. pseudohaemuli<\/jats:italic> based on genomic data.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.70025","type":"journal-article","created":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T11:34:10Z","timestamp":1737632050000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular Epidemiology and Antifungal Susceptibility Profile of <i>Candidozyma<\/i> Isolates From Argentina"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6985-2699","authenticated-orcid":false,"given":"Constanza Giselle","family":"Taverna","sequence":"first","affiliation":[{"name":"Departamento Micolog\u00eda\u2014Instituto Nacional de Enfermedades Infecciosas \u201cDr. Carlos G. Malbr\u00e1n\u201d  Buenos Aires Ciudad Aut\u00f3noma de Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"C\u00f3rdoba","sequence":"additional","affiliation":[{"name":"Departamento Micolog\u00eda\u2014Instituto Nacional de Enfermedades Infecciosas \u201cDr. Carlos G. Malbr\u00e1n\u201d  Buenos Aires Ciudad Aut\u00f3noma de Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Sol","family":"Haim","sequence":"additional","affiliation":[{"name":"Unidad Operativa Centro Nacional de Gen\u00f3mica y Bioinform\u00e1tica\u2014ANLIS \u201cDr. Carlos G. Malbr\u00e1n\u201d  Buenos Aires Ciudad Aut\u00f3noma de Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micaela","family":"Lombardo","sequence":"additional","affiliation":[{"name":"Departamento Micolog\u00eda\u2014Instituto Nacional de Enfermedades Infecciosas \u201cDr. Carlos G. Malbr\u00e1n\u201d  Buenos Aires Ciudad Aut\u00f3noma de Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mat\u00edas Ezequiel","family":"Vivot","sequence":"additional","affiliation":[{"name":"Departamento Micolog\u00eda\u2014Instituto Nacional de Enfermedades Infecciosas \u201cDr. Carlos G. Malbr\u00e1n\u201d  Buenos Aires Ciudad Aut\u00f3noma de Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e1rbara Abigail","family":"Arias","sequence":"additional","affiliation":[{"name":"Departamento Micolog\u00eda\u2014Instituto Nacional de Enfermedades Infecciosas \u201cDr. Carlos G. Malbr\u00e1n\u201d  Buenos Aires Ciudad Aut\u00f3noma de Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter","family":"Vivot","sequence":"additional","affiliation":[{"name":"Departamento Micolog\u00eda\u2014Instituto Nacional de Enfermedades Infecciosas \u201cDr. Carlos G. Malbr\u00e1n\u201d  Buenos Aires Ciudad Aut\u00f3noma de Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanda","family":"Szusz","sequence":"additional","affiliation":[{"name":"Departamento Micolog\u00eda\u2014Instituto Nacional de Enfermedades Infecciosas \u201cDr. Carlos G. Malbr\u00e1n\u201d  Buenos Aires Ciudad Aut\u00f3noma de Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4667-8004","authenticated-orcid":false,"given":"Darren","family":"Abbey","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0384-6066","authenticated-orcid":false,"given":"Tomas Javier","family":"Pokl\u00e9povich","sequence":"additional","affiliation":[{"name":"Unidad Operativa Centro Nacional de Gen\u00f3mica y Bioinform\u00e1tica\u2014ANLIS \u201cDr. Carlos G. Malbr\u00e1n\u201d  Buenos Aires Ciudad Aut\u00f3noma de Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina Elena","family":"Canteros","sequence":"additional","affiliation":[{"name":"Departamento Micolog\u00eda\u2014Instituto Nacional de Enfermedades Infecciosas \u201cDr. Carlos G. Malbr\u00e1n\u201d  Buenos Aires Ciudad Aut\u00f3noma de Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02248-12"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2006.tb03816.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2008.00083.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001302"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myad009"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-013-1313-5"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-010-9451-3"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-008-9238-y"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0240093"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2008.00372.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof5030058"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3767\/persoonia.2024.52.02"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S402754"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2903.221082"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00084"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1130645"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-018-0338-9"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuz015"},{"key":"e_1_2_9_20_1","first-page":"100","article-title":"YMAP: A Pipeline for Visualization of Copy Number Variation and Loss of Heterozygosity in Eukaryotic Pathogens","volume":"6","author":"Abbey D. A.","year":"2014","journal-title":"Genome Medicine"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msad009"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01777-22"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.15698\/mic2022.11.786"},{"key":"e_1_2_9_24_1","article-title":"Whole Genome Sequencing of Candida Glabrata for Detection of Markers of Antifungal Drug Resistance","volume":"130","author":"Biswas C.","year":"2017","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2016.07.003"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw691"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2509.190686"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30597-8"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00577-23"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2704.204361"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01146-21"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00842-22"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.03333-20"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07779-6"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.00554"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myad030"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof6030138"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S118892"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix131"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.0c00199"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkac161"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12281-017-0300-y"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1590\/0074-0276108022013009"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2014-15-3-r46"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2417-3_17"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00176-18"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00204-18"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx248"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp698"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giab008"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1754"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-52149-1.00013-6"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1055-7903(03)00208-2"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2024.102491"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.3389\/ffunb.2022.1063609"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.1.77-84.2006"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.4137\/EBO.S653"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.47371\/mycosci.MYC47065"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-1356(03)00113-2"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00222-07"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0011453"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myx005"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof8060574"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1001845"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myy021"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2005.02683.x"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01474-23"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02512-19"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00423-08"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01145-12"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2023.04.025"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.11.024"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2024.2398596"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13100-020-00215-x"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.15739260"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.60474"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.3389\/ffunb.2021.729264"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.70025","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T09:10:46Z","timestamp":1748423446000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.70025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":76,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/myc.70025"],"URL":"https:\/\/doi.org\/10.1111\/myc.70025","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-09-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70025"}}