{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:16:26Z","timestamp":1787015786779,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T00:00:00Z","timestamp":1744675200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2025,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n \n Pulmonary aspergillosis is a major global health concern, yet its diagnosis remains challenging.\n Aspergillus<\/jats:italic>\n \u2010specific IgG measurement is essential for identifying chronic and allergic forms.\n <\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n \n This study aimed to evaluate a quantitative method, the ImmunoCAP assay IgG m3 (ICAP) (Phadia\u2010ThermoFisher Scientific, Waltham, USA), a qualitative method, the\n Aspergillus<\/jats:italic>\n IgG Western blot kit (Asp\u2010WB) (LDBio Diagnostics, Lyon, France) and a combination of both methods for the diagnosis of\n Aspergillus<\/jats:italic>\n lung disease.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n A retrospective study was conducted at the University Hospital of Marseille, France, during 1\u2009year. Patients undergoing\n Aspergillus<\/jats:italic>\n serology were divided into three groups: Group 1 (G1) with ICAP \u2265\u200940\u2009mgA\/L and positive Asp\u2010WB, Group 2 (G2) with ICAP \u2265\u200940\u2009mgA\/L and negative Asp\u2010WB and Group 3 (G3) with ICAP <\u200940 mgA\/L and positive Asp\u2010WB. Data were collected on demographics, underlying diseases, imaging and biological outcomes. Patients were classified according to their\n Aspergillus<\/jats:italic>\n lung disease, whether acute pulmonary aspergillosis, chronic pulmonary aspergillosis (CPA), allergic broncho\u2010pulmonary aspergillosis (ABPA), colonisation or\n Aspergillus<\/jats:italic>\n sensitisation.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n A total of 536 patients were studied: 173 in G1, 204 in G2 and 200 in G3, with 38 patients found in several groups. The primary underlying disease was cystic fibrosis in 44.6% of patients. Twenty\u2010two patients were diagnosed with ABPA. The number of diagnosed ABPA cases in G1 (20; 11.6%) combining positive ICAP and Asp\u2010WB was significantly higher than that found in the groups with a single positive test result (\n p<\/jats:italic>\n \u2009<\u20090.001). Fifteen patients were diagnosed with CPA. Isolated positive Western blot (G3) identified five cases of aspergilloma. Significantly fewer\n Aspergillus<\/jats:italic>\n lung diseases were diagnosed in isolated positive ICAP G2 (8.8%) than in G1 (53.8%) and G3 (42.5%) (\n p<\/jats:italic>\n \u2009<\u20090.001).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n This study highlights the benefits of combining Asp\u2010WB and ICAP for the diagnosis of\n Aspergillus<\/jats:italic>\n lung disease and the relatively high false\u2010positive rate in patients with isolated positive ICAP results.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/myc.70058","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T07:44:56Z","timestamp":1744703096000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Combined\n ImmunoCAP<\/scp>\n and Western Blot for the Diagnosis of\n Aspergillus<\/i>\n Lung Disease"],"prefix":"10.1111","volume":"68","author":[{"given":"Emanuel","family":"Petit","sequence":"first","affiliation":[{"name":"AP\u2010HM Laboratoires Des Agents Infectieux Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Cortaredona","sequence":"additional","affiliation":[{"name":"IHU M\u00e9diterran\u00e9e Infection Marseille France"},{"name":"Aix\u2010Marseille Universit\u00e9 SSA, RITMES Marseille France"},{"name":"Aix\u2010Marseille Univ, IRD SSA, Mines Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Sicco","sequence":"additional","affiliation":[{"name":"AP\u2010HM Laboratoires Des Agents Infectieux Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3293-5276","authenticated-orcid":false,"given":"St\u00e9phane","family":"Ranque","sequence":"additional","affiliation":[{"name":"AP\u2010HM Laboratoires Des Agents Infectieux Marseille France"},{"name":"IHU M\u00e9diterran\u00e9e Infection Marseille France"},{"name":"Aix\u2010Marseille Universit\u00e9 SSA, RITMES Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0681-8431","authenticated-orcid":false,"given":"Estelle","family":"Menu","sequence":"additional","affiliation":[{"name":"AP\u2010HM Laboratoires Des Agents Infectieux Marseille France"},{"name":"IHU M\u00e9diterran\u00e9e Infection Marseille France"},{"name":"Aix\u2010Marseille Universit\u00e9 SSA, RITMES Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00140\u201018"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof3040057"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00583\u20102015"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01810"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12141"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2020.08.043"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.08.029"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12815"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2015.11.003"},{"issue":"58","key":"e_1_2_9_11_1","first-page":"4644","article-title":"Comparison of 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