{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:15:35Z","timestamp":1789172135698,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,3]],"date-time":"2025-05-03T00:00:00Z","timestamp":1746230400000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      This study aimed to systematically evaluate and compare the performance of two laboratory\u2010developed assays (LDAs) and three commercially available real\u2010time PCR assays for the detection of\n                      <jats:italic>Candida auris<\/jats:italic>\n                      . The analytical sensitivity, specificity and limit of detection (LOD) of each assay were assessed, alongside their clinical sensitivity in identifying\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C. auris<\/jats:italic>\n                      <\/jats:styled-content>\n                      colonisation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Ten\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C. auris<\/jats:italic>\n                      <\/jats:styled-content>\n                      strains representing five clades, as well as genetically related yeasts, common yeast species, and dermatophytes, were used to assess assay sensitivity and cross reactivity. Clinical and environmental samples were collected from patients during an outbreak and tested with three commercial PCR assays (AurisID, Fungiplex, FungiXpert) and two LDAs (CDC LDA, EMC LDA). LOD was determined using Probit analysis. Diagnostic sensitivity was evaluated by comparing the detection rate of each individual assay to the total detection rate of all assays combined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The EMC LDA exhibited the highest analytical sensitivity, with a LOD of 8 conidia\/reaction, followed by CDC LDA (16 conidia\/reaction), AurisID and FungiXpert (19 conidia\/reaction), and Fungiplex (596 conidia\/reaction). Specificity testing revealed cross\u2010reactivity in the CDC LDA and AurisID assays with\n                      <jats:italic>C. pseudohaemulonii<\/jats:italic>\n                      at high conidia levels, while no cross\u2010reactivity was observed in the other assays. EMC LDA showed the highest clinical sensitivity (100%), whereas Fungiplex had the lowest positivity rate (71%). No false positives were observed in negative control swabs for any assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Real\u2010time PCR is a crucial tool for the rapid and sensitive detection of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C. auris<\/jats:italic>\n                      <\/jats:styled-content>\n                      , especially in clinical settings where timely identification is essential for effective patient management and infection control. Numerous PCR assays are available for this purpose; however, our study demonstrates that the sensitivity of these assays can vary significantly. The observed differences underscore the importance of establishing international reference standards and proficiency panels to enhance the accuracy and comparability of assay performance across different studies and laboratories.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.70065","type":"journal-article","created":{"date-parts":[[2025,5,3]],"date-time":"2025-05-03T06:42:57Z","timestamp":1746254577000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Performance Evaluation of Five Real\u2010Time\n                    <scp>PCR<\/scp>\n                    Assays for the Detection of\n                    <i>Candida auris<\/i>\n                    <scp>DNA<\/scp>"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4031-0778","authenticated-orcid":false,"given":"Jochem B.","family":"Buil","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Radboudumc  Nijmegen the Netherlands"},{"name":"Radboudumc\u2010CWZ Center of Expertise for Mycology  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart","family":"van den Bosch","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Radboudumc  Nijmegen the Netherlands"},{"name":"Radboudumc\u2010CWZ Center of Expertise for Mycology  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzan J.","family":"van der Maas","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Radboudumc  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eelco F. J.","family":"Meijer","sequence":"additional","affiliation":[{"name":"Radboudumc\u2010CWZ Center of Expertise for Mycology  Nijmegen the Netherlands"},{"name":"Department of Medical Microbiology and Immunology Canisius Wilhelmina Hospital  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theun","family":"de Groot","sequence":"additional","affiliation":[{"name":"Radboudumc\u2010CWZ Center of Expertise for Mycology  Nijmegen the Netherlands"},{"name":"Department of Medical Microbiology and Immunology Canisius Wilhelmina Hospital  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8869-1509","authenticated-orcid":false,"given":"Joseph","family":"Meletiadis","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory Attikon University Hospital, Medical School, National and Kapodistrian University of Athens  Athens Greece"},{"name":"Department of Medical Microbiology and Infectious Diseases Erasmus MC  Rotterdam the Netherlands"},{"name":"Unit for Mycology Statens Serum Institute  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul E.","family":"Verweij","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Radboudumc  Nijmegen the Netherlands"},{"name":"Radboudumc\u2010CWZ Center of Expertise for Mycology  Nijmegen the Netherlands"},{"name":"Center for Infectious Disease Research, Diagnostics and Laboratory Surveillance National Institute for Public Health and the Environment (RIVM), Bilthoven  the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem J. G.","family":"Melchers","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Radboudumc  Nijmegen the Netherlands"},{"name":"Radboudumc\u2010CWZ Center of Expertise for Mycology  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzan D.","family":"Pas","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Radboudumc  Nijmegen the Netherlands"},{"name":"Radboudumc\u2010CWZ Center of Expertise for Mycology  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1910.130393"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2007.131765"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2410.180649"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2008.00083.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006290"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw691"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2509.190686"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.03364-19"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(24)00101-0"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciab327"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00921-17"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9040807"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof9070715"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-013-2027-1"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01223-17"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011698"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2023.12.019"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7020154"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01503\u201019"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.70065","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/myc.70065","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.70065","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:58:19Z","timestamp":1789171099000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.70065"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1111\/myc.70065"],"URL":"https:\/\/doi.org\/10.1111\/myc.70065","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"2024-12-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70065"}}