{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T23:01:08Z","timestamp":1772665268553,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T00:00:00Z","timestamp":1748822400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Due to the paucity of fungal elements in the tissue, the sensitivity of histopathological tests for the diagnosis of cutaneous sporotrichosis remains low, particularly for low\u2010virulent <jats:italic>Sporothrix<\/jats:italic> species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We retrospectively analysed and re\u2010evaluated the role of histopathological examination in the diagnosis of cutaneous sporotrichosis caused by <jats:italic>Sporothrix globosa<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Retrospective analysis and pathological reassessment were conducted for 69 sporotrichosis cases caused by <jats:styled-content style=\"fixed-case\"><jats:italic>S. globosa<\/jats:italic><\/jats:styled-content> between 2013 and 2024. Statistical analysis using prevalence ratios was conducted to characterise the clinicopathological and epidemiological aspects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Histopathological examination revealed mixed inflammatory cell infiltration and granulomatous changes in all cases. Periodic acid\u2010Schiff (PAS) staining was performed in 29 tissue specimens, with positivity observed in 4 cases (13.8%, 4\/29). Following supplemental staining and re\u2010evaluation of the slides, PAS staining revealed positivity in 50.7% (35\/69) of cases, demonstrating yeast forms (91.4%, 32\/35) with asteroid bodies (<jats:italic>n<\/jats:italic>\u2009=\u20092) and rare hyphae (<jats:italic>n<\/jats:italic>\u2009=\u20091).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Currently, the role of histopathological examination in the diagnosis of sporotrichosis is limited. Our study showed that the frequency of detecting <jats:styled-content style=\"fixed-case\"><jats:italic>S.\u2009globosa<\/jats:italic><\/jats:styled-content> based on histopathological examination is considerably high. Nevertheless, achieving high positive rates necessitates the collaborative efforts of experienced mycologists. The predominance of suppurative granulomas or neutrophils is related to the presence of the fungus in tissue sections from human patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.70072","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T01:55:02Z","timestamp":1748915702000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Role of Histopathological Examination in the Diagnosis of Cutaneous Sporotrichosis Caused by <i>Sporothrix globosa<\/i>: A Retrospective Analysis and Reassessment"],"prefix":"10.1111","volume":"68","author":[{"given":"Liuyu","family":"Li","sequence":"first","affiliation":[{"name":"Department of Dermatology Hangzhou Third People's Hospital, Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiajia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hangzhou Third People's Hospital, Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zehu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hangzhou Third People's Hospital, Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3790-8026","authenticated-orcid":false,"given":"Xiujiao","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hangzhou Third People's Hospital, Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.riam.2018.09.002"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00808-07"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780801992837"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof4030095"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1590\/abd1806-4841.2017279"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S354380"},{"issue":"5","key":"e_1_2_11_8_1","article-title":"Emerging Sporotrichosis Is Driven by Clonal and Recombinant Sporothrix Species","volume":"3","author":"Rodrigues A. 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