{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:43:34Z","timestamp":1790012614351,"version":"4.0.1"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:00:00Z","timestamp":1751587200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["408861\/2022\u20103"],"award-info":[{"award-number":["408861\/2022\u20103"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["310414\/2022\u20109"],"award-info":[{"award-number":["310414\/2022\u20109"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Candida<\/jats:italic>\n                      (\n                      <jats:italic>Candidozyma<\/jats:italic>\n                      )\n                      <jats:italic>auris<\/jats:italic>\n                      has distinct genetic clades. Clade distinction is relevant for infection control and epidemiological purposes. State\u2010of\u2010the\u2010art typing methodologies for clade distinction are based on genomic approaches, such as short tandem repeat (STR) analysis and whole\u2010genome sequencing (WGS). However, they are time\u2010consuming and expensive. Fourier transform infrared spectroscopy (FT\u2010IR) is an alternative tool for strain typing based on their unique biochemical spectral profiles.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To apply FT\u2010IR to differentiate\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C. auris<\/jats:italic>\n                      <\/jats:styled-content>\n                      clades and evaluate epidemiological relationships based on biochemical data among isolates from a multicenter\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C. auris<\/jats:italic>\n                      <\/jats:styled-content>\n                      outbreak in the state of Pernambuco, northeastern Brazil.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Sixty\u2010nine\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C. auris<\/jats:italic>\n                      <\/jats:styled-content>\n                      strains from clades I, II, III, and IV were analysed. Fifty\u2010nine were clade IV strains obtained from three outbreaks that took place in Pernambuco state, northeastern Brazil. An adjusted FT\u2010IR spectroscopy protocol was applied to obtain carbohydrates and lipid fingerprints. Short Tandem Repeat (STR) analysis was used in order to validate the spectroscopy approach.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The adjusted preparation protocol for FT\u2010IR analysis improved the spectral quality by 31.42% compared to standard protocol. FT\u2010IR allowed us to discriminate\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C. auris<\/jats:italic>\n                      <\/jats:styled-content>\n                      clades I to IV. Moreover, important similarities were observed in\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C. auris<\/jats:italic>\n                      <\/jats:styled-content>\n                      clade IV strains obtained from two of the three hospitals, based on polysaccharides (1300\u2013800\u2009cm\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) plus lipids (3000\u20132800\u2009cm\n                      <jats:sup>\u22121<\/jats:sup>\n                      and 1500\u20131400\u2009cm\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) spectra. STR confirmed the similarity results obtained by FT\u2010IR, clustering the strains from two different hospitals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The IR Biotyper is fast, easy\u2010to\u2010use, and a promising alternative for moderate\u2010to\u2010high\u2010complexity laboratories to differentiate\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C. auris<\/jats:italic>\n                      <\/jats:styled-content>\n                      clades. Furthermore, this technique has the potential for isolate\u2010level source tracking, which could be valuable for monitoring transmission routes in clinical settings.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.70085","type":"journal-article","created":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T03:39:49Z","timestamp":1751600389000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Clade Distinction and Tracking of Clonal Spread by Fourier\u2010Transform Infrared Spectroscopy in Multicenter\n                    <i>Candida<\/i>\n                    (\n                    <i>Candidozyma<\/i>\n                    )\n                    <i>auris<\/i>\n                    Outbreak"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5370-7037","authenticated-orcid":false,"given":"Camylla C.","family":"de Melo","sequence":"first","affiliation":[{"name":"Laboratory for Research and Diagnosis of Tropical Diseases, Center for Medical Sciences Federal University of Pernambuco (UFPE)  Recife Brazil"},{"name":"Post\u2010Graduation Program in Tropical Medicine Federal University of Pernambuco (UFPE)  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halana L. N. L.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory for Research and Diagnosis of Tropical Diseases, Center for Medical Sciences Federal University of Pernambuco (UFPE)  Recife Brazil"},{"name":"Post\u2010Graduation Program in Tropical Medicine Federal University of Pernambuco (UFPE)  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna R.","family":"Souza","sequence":"additional","affiliation":[{"name":"Laboratory for Research and Diagnosis of Tropical Diseases, Center for Medical Sciences Federal University of Pernambuco (UFPE)  Recife Brazil"},{"name":"Post\u2010Graduation Program in Tropical Medicine Federal University of Pernambuco (UFPE)  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla V. R.","family":"Moura","sequence":"additional","affiliation":[{"name":"Laboratory for Research and Diagnosis of Tropical Diseases, Center for Medical Sciences Federal University of Pernambuco (UFPE)  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Bruker Daltonics GmbH &amp; Co. KG  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6781-9617","authenticated-orcid":false,"given":"Rafael W.","family":"Bastos","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology Federal University of Rio Grande Do Norte  Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karoline Kristina","family":"Kemmerich","sequence":"additional","affiliation":[{"name":"Department of Medicine Federal University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3766-026X","authenticated-orcid":false,"given":"Jo\u00e3o N.","family":"de Almeida\u2010J\u00fanior","sequence":"additional","affiliation":[{"name":"Department of Medicine Federal University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Antimicrobial Resistance Institute of S\u00e3o Paulo (ARIES)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0793-8491","authenticated-orcid":false,"given":"Arnaldo Lopes","family":"Colombo","sequence":"additional","affiliation":[{"name":"Department of Medicine Federal University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Antimicrobial Resistance Institute of S\u00e3o Paulo (ARIES)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bram","family":"Spruijtenburg","sequence":"additional","affiliation":[{"name":"Radboudumc \u2013 CWZ Center of Expertise for Mycology  Nijmegen the Netherlands"},{"name":"Department of Medical Microbiology and Immunology Canisius\u2010Wihelmina Hospital (CWZ)\/Diccon  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3253-6080","authenticated-orcid":false,"given":"Jacques F.","family":"Meis","sequence":"additional","affiliation":[{"name":"Radboudumc \u2013 CWZ Center of Expertise for Mycology  Nijmegen the Netherlands"},{"name":"Institute of Translational Research, Cologne Excellence Cluster on Cellular Stress Responses in Aging\u2010Associated Diseases (CECAD) University of Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Le Pape","sequence":"additional","affiliation":[{"name":"Cibles et M\u00e9dicaments Des Infections et de L'immunit\u00e9, IICiMed, UR 1155 Universit\u00e9 de Nantes  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn G. 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