{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T15:45:06Z","timestamp":1780587906018,"version":"3.54.1"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Small studies have used various serological methods to evaluate the response to paracoccidioidomycosis (PCM) treatment, with limited use of counterimmunoelectrophoresis (CIE). This study assessed CIE titres during and after PCM therapy and their prognostic value for serological negativity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this retrospective study, we reviewed medical records of patients with positive serology in proven or probable PCM from 2006 to 2021 at University of S\u00e3o Paulo. We performed multivariate logistic regression to identify independent variables associated with CIE titre negativity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      This study included 144 participants, totalling 979 serology samples analysed, with a predominance of middle\u2010aged adults (median age 50\u2009years), males (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009112, 78%) and chronic form (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009112, 78%). Trimethoprim\u2010sulfamethoxazole (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200979, 55%) and itraconazole (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200955, 38%) were the drugs most commonly used. The median treatment time was 24\u2009months (IQR 16\u201337). Median initial CIE titre was 1:32 (IQR 1:16\u20131:128). Thirty\u2010seven patients (26%) had a negative CIE titre, and 105 patients (73%) had CIE titres \u2264\u20091:4 at the last medical appointment. In multivariate analysis, only positive direct microscopy examination (OR 0.32,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.043) was an independent factor related to non\u2010negativity serology. The time to negativity was shorter in female sex and negative microscopy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The serology using CIE presented a strong association with clinical response, making it a valuable method for monitoring patients with PCM. Most patients achieved CIE titres \u2264\u20091:4 during antifungal therapy, which was strongly associated with a successful clinical response.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.70096","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T06:19:07Z","timestamp":1753251547000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Serological Response and Associated Prognostic Factors in Paracoccidioidomycosis: A 15\u2010Year Retrospective Study"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7094-3440","authenticated-orcid":false,"given":"Wdson Luis Lima","family":"Kruschewsky","sequence":"first","affiliation":[{"name":"Infectious Diseases and Tropical Medicine Department Hospital das Cl\u00ednicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo 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