{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:41:45Z","timestamp":1791906105558,"version":"4.3.4"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013314","name":"Higher Education Discipline Innovation Project","doi-asserted-by":"publisher","award":["D20009"],"award-info":[{"award-number":["D20009"]}],"id":[{"id":"10.13039\/501100013314","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32460051"],"award-info":[{"award-number":["32460051"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Geophilic <jats:italic>Nannizzia<\/jats:italic> dermatophytes are increasingly implicated in stubborn skin, hair, and nail infections, yet MALDI\u2010TOF MS evaluations and antifungal\u2010susceptibility data have focused almost exclusively on <jats:italic>N. gypsea<\/jats:italic>. Biochemical profiles and MICs cut\u2010offs are limited.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To benchmark two commercial MALDI\u2010TOF MS libraries and to determine in\u00a0vitro activity of eight antifungals against a genus\u2010wide panel of <jats:italic>Nannizzia<\/jats:italic> species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>One\u2010hundred\u2010and\u2010three ITS\u2010confirmed isolates representing 12 species were grown on potato\u2010dextrose agar (PDA) for 7\u201314\u2009days. Spectra were acquired with (i) the MSI\u20102 Dermatophyte Library after 4\u201314\u2009days' PDA incubation (100 cultures) and (ii) the Bruker MALDI Biotyper Filamentous\u2010Fungi Library 6.0\/2023 after \u2264\u20093\u2009days' growth in Sabouraud\u2010dextrose broth (SDB) (73 cultures). BCCM\/IHEM strains could not be evaluated on the Biotyper because of licence restrictions, leaving 73 non\u2010duplicate isolates for direct MSI\u20102 vs MBT comparison. EUCAST E.Def 11.0 micro\u2010broth dilution determined MICs for eight agents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MSI\u20102 achieved its highest accuracy with PDA day\u20107 cultures (45\/73, 62%), whereas the liquid Biotyper protocol yielded 49\/73 correct identifications (67%) within four days. Accepting low\u2010confidence scores (\u2265\u20091.7) from either library increased overall accuracy to 73%. MSI\u20102 remained superior for <jats:italic>N. gypsea<\/jats:italic> (73%) and uniquely detected <jats:italic>N. nana<\/jats:italic> (50%), which is absent from the current Biotyper release. Conversely, the Biotyper outperformed MSI\u20102 for <jats:italic>N. incurvata<\/jats:italic>, <jats:italic>N. fulva<\/jats:italic>, and <jats:italic>N. praecox<\/jats:italic>. Six very rare species (<jats:italic>N. lorica<\/jats:italic>, <jats:italic>N. aenigmatica<\/jats:italic>, <jats:italic>N. corniculata<\/jats:italic>, <jats:italic>N. duboisii<\/jats:italic>, <jats:italic>N. perplicata<\/jats:italic>, <jats:italic>N. polymorpha<\/jats:italic>) were not recognised by either database. Terbinafine displayed the lowest geometric mean MIC (0.009\u2009mg\/L); fluconazole and griseofulvin showed the highest values, and one US <jats:italic>N. fulva<\/jats:italic> isolate exhibited elevated itraconazole\/voriconazole MICs (1\u2009mg\/L).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Diagnostic coverage of <jats:italic>Nannizzia<\/jats:italic> remains incomplete. Expanding commercial MALDI\u2010ToF MS libraries with spectra from rare species and performing routine susceptibility testing are essential to optimise patient management.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.70117","type":"journal-article","created":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T15:32:35Z","timestamp":1758209555000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Utility of <scp>MALDI<\/scp>\u2010<scp>ToF MS<\/scp> for Recognition and Antifungal Susceptibility of <i>Nannizzia<\/i>, an Underestimated Group of Dermatophytes"],"prefix":"10.1111","volume":"68","author":[{"given":"Chao","family":"Tang","sequence":"first","affiliation":[{"name":"Guizhou Key Laboratory of Microbiome and Infectious Disease Prevention and Control &amp; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou &amp; School of Basic Medical Science &amp; Institution of One Health Research Guizhou Medical University  Guiyang China"},{"name":"Center of Expertise in Mycology Radboud University Medical Center\/Canisius Wilhelmina Hospital  Nijmegen the Netherlands"},{"name":"Foundation Atlas of Clinical Fungi  Hilversum the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology, Institute of Dermatology Chinese Academy of Medical Science &amp; Peking Union Medical College  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmijn","family":"Jansen","sequence":"additional","affiliation":[{"name":"Saxion University of Applied Sciences  Apeldoorn the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Vossgroene","sequence":"additional","affiliation":[{"name":"Bruker Daltonics GmbH &amp; Co. KG  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi\u2010Lam\u2010An","family":"Vu","sequence":"additional","affiliation":[{"name":"Bruker Daltonics GmbH &amp; Co. KG  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Oberheitmann","sequence":"additional","affiliation":[{"name":"Bruker Daltonics GmbH &amp; Co. KG  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlou","family":"Tehupeiory\u2010Kooreman","sequence":"additional","affiliation":[{"name":"Center of Expertise in Mycology Radboud University Medical Center\/Canisius Wilhelmina Hospital  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoqin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guizhou Key Laboratory of Microbiome and Infectious Disease Prevention and Control &amp; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou &amp; School of Basic Medical Science &amp; Institution of One Health Research Guizhou Medical University  Guiyang China"},{"name":"Center of Expertise in Mycology Radboud University Medical Center\/Canisius Wilhelmina Hospital  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Center of Expertise in Mycology Radboud University Medical Center\/Canisius Wilhelmina Hospital  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clement Kin\u2010ming","family":"Tsui","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Laboratory National Centre for Infectious Diseases  Singapore"},{"name":"Lee Kong Chian School of Medicine Nanyang Technological University  Singapore"},{"name":"Faculty of Medicine University of British Columbia  Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weida","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology, Institute of Dermatology Chinese Academy of Medical Science &amp; Peking Union Medical College  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingqian","family":"Kang","sequence":"additional","affiliation":[{"name":"Guizhou Key Laboratory of Microbiome and Infectious Disease Prevention and Control &amp; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou &amp; School of Basic Medical Science &amp; Institution of One Health Research Guizhou Medical University  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0155-8113","authenticated-orcid":false,"given":"Sarah A.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Center of Expertise in Mycology Radboud University Medical Center\/Canisius Wilhelmina Hospital  Nijmegen the Netherlands"},{"name":"Foundation Atlas of Clinical Fungi  Hilversum the Netherlands"},{"name":"Department of Microbiology, Faculty of Medicine Kuwait University  Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5344-257X","authenticated-orcid":false,"given":"Sybren","family":"de Hoog","sequence":"additional","affiliation":[{"name":"Guizhou Key Laboratory of Microbiome and Infectious Disease Prevention and Control &amp; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou &amp; School of Basic Medical Science &amp; Institution of One Health Research Guizhou Medical University  Guiyang China"},{"name":"Center of Expertise in Mycology Radboud University Medical Center\/Canisius Wilhelmina Hospital  Nijmegen the Netherlands"},{"name":"Foundation Atlas of Clinical Fungi  Hilversum the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.3c01988"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-016-0073-9"},{"key":"e_1_2_9_4_1","first-page":"e296","article-title":"Tinea Capitis by Microsporum gypseum, an Infrequent Species","volume":"116","author":"Garc\u00eda\u2010Agudo L. 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