{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:51:18Z","timestamp":1790718678455,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T00:00:00Z","timestamp":1771027200000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100013068","name":"Key Technologies Research and Development Program of Guangzhou Municipality","doi-asserted-by":"publisher","award":["2024B01J1191"],"award-info":[{"award-number":["2024B01J1191"]}],"id":[{"id":"10.13039\/501100013068","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U24A20745"],"award-info":[{"award-number":["U24A20745"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2020T130151ZX"],"award-info":[{"award-number":["2020T130151ZX"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Conventional diagnostic methods for onychomycosis possess disadvantages related to limited sensitivity, extended turnaround times and strong reliance on operator expertise.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To develop a molecular assay with high sensitivity and specificity for rapid detection of multiple fungal pathogens in nail specimens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A multi\u2010tube recombinase polymerase amplification (RPA) assay with fluorescent signal detection was developed, targeting pan\u2010fungal,\n                      <jats:italic>Trichophyton rubrum<\/jats:italic>\n                      ,\n                      <jats:italic>T.\u2009interdigitale<\/jats:italic>\n                      , pan\u2010\n                      <jats:italic>Candida<\/jats:italic>\n                      ,\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Candida albicans<\/jats:italic>\n                      <\/jats:styled-content>\n                      , pan\u2010\n                      <jats:italic>Trichosporon<\/jats:italic>\n                      and pan\u2010\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      . The analytical sensitivity and specificity of the assay were evaluated using fungal strains, and its performance was assessed in 81 clinical nail specimens in parallel with calcofluor white fluorescence microscopy and fungal culture.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The multi\u2010tube RPA assay demonstrated a detection limit of 1\u2009pg of genomic DNA for all targets, with a turnaround time of &lt;\u200970\u2009min. In the evaluation of 81 clinical nail specimens, Bayesian latent class analysis estimated sensitivities of 86.0% for multi\u2010tube RPA, 67.0% for fungal culture and 77.0% for microscopy, with corresponding specificities of 85.0%, 89.0% and 87.0%. The positive and negative predictive values were 92.0% and 72.0% for multi\u2010tube RPA, 93.0% and 55.0% for fungal culture, and 93.0% and 66.0% for microscopy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Compared with fungal culture and microscopy, the multi\u2010tube RPA assay demonstrated superior sensitivity while maintaining good specificity, facilitating the identification of fungal pathogens at the species level in nail specimens. Its operational simplicity, enhanced diagnostic performance and reduced processing times make it a promising alternative for the mycological diagnosis of onychomycosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.70162","type":"journal-article","created":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T10:02:45Z","timestamp":1771063365000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Rapid Detection of Fungi in Nail Specimens Using Multi\u2010Tube Recombinase Polymerase Amplification Assay"],"prefix":"10.1111","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2153-9182","authenticated-orcid":false,"given":"Rong","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Dermatology, The Third Affiliated Hospital Sun Yat\u2010Sen University  Guangzhou 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Sun Yat\u2010Sen University  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Medical Research Center, Sun Yat\u2010Sen Memorial Hospital Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaiqiu","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The Third Affiliated Hospital Sun Yat\u2010Sen University  Guangzhou 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