{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T11:00:16Z","timestamp":1785236416485,"version":"3.55.0"},"reference-count":51,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100004319","name":"Pfizer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004319","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n \n The black moulds comprising\n Aspergillus<\/jats:italic>\n section\n Nigri<\/jats:italic>\n are clinically important organisms causing aspergillosis and otomycosis; triazole (azole) antifungal agents represent the primary prophylaxis and treatment option for these infections. The cryptic nature of species within section\n Nigri<\/jats:italic>\n presents challenges for routine identification methods, yet accurate speciation may reveal important and overlooked trends in species distribution and azole antifungal activity.\n <\/jats:p>\n <\/jats:sec>\n \n Method<\/jats:title>\n \n We used whole genome sequencing and comparative genomics to speciate 135 clinical\n A.<\/jats:italic>\n section\n Nigri<\/jats:italic>\n isolates collected worldwide from 2020 to 2023 and broth microdilution susceptibility testing to assess antifungal agent activity.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n A<\/jats:italic>\n .\n welwitschiae<\/jats:italic>\n (\n n\u2009=<\/jats:italic>\n \u200961; 45.2%) and\n A<\/jats:italic>\n .\n tubingensis<\/jats:italic>\n (\n n\u2009=<\/jats:italic>\n \u200951; 37.8%) were the primary species identified.\n A<\/jats:italic>\n .\n tubingensis<\/jats:italic>\n accounted for 87.9% (\n n\u2009=<\/jats:italic>\n \u200929\/33) of isolates producing azole MIC values above the CLSI\u2010established\n \n A<\/jats:italic>\n .\n niger<\/jats:italic>\n <\/jats:styled-content>\n epidemiological cutoff values (ECV). All azoles displayed high activity (\u2265\u200993.7% wildtype [WT]) against\n A<\/jats:italic>\n .\n welwitschiae<\/jats:italic>\n and\n \n A<\/jats:italic>\n .\n niger<\/jats:italic>\n <\/jats:styled-content>\n isolates;\n A<\/jats:italic>\n .\n tubingensis<\/jats:italic>\n isolates were broadly inhibited by posaconazole (100% WT). The frequency of\n A<\/jats:italic>\n .\n tubingensis<\/jats:italic>\n isolates with NWT phenotypes against \u2265\u20092 azoles and the modal MIC values for most agents increased over time; azole\u2010NWT isolates were present across all regions. Comparisons of protein sequences of CYP51A and CYP51B, the molecular target of azole activity, revealed unique residues in\n A<\/jats:italic>\n .\n tubingensis<\/jats:italic>\n . However, most azole\u2010NWT isolates across species lacked unique CYP51A\/B alterations, suggesting mechanisms outside target site modification influence elevated azole MIC values in this section.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Our results demonstrate the utility of speciating clinically relevant organism groups to better understand the dynamics of antifungal agent activity and species distributions.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/myc.70197","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T06:51:32Z","timestamp":1783493492000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reduced Activity of Triazole Antifungal Agents Against\n Aspergillus tubingensis<\/i>\n From a Global Collection of Genomically\u2010Characterized\n Aspergillus<\/i>\n Section\n Nigri<\/i>\n Clinical Isolates (2020\u20132023)"],"prefix":"10.1111","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0457-1543","authenticated-orcid":false,"given":"John H.","family":"Kimbrough","sequence":"first","affiliation":[{"name":"Element Iowa City (JMI Laboratories) North Liberty Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9785-5034","authenticated-orcid":false,"given":"Marisa L.","family":"Winkler","sequence":"additional","affiliation":[{"name":"Element Iowa City (JMI Laboratories) North Liberty Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maura H.","family":"Karr","sequence":"additional","affiliation":[{"name":"Element Iowa City (JMI Laboratories) North Liberty Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica M.","family":"Ewald","sequence":"additional","affiliation":[{"name":"Element Iowa City (JMI Laboratories) North Liberty Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Castanheira","sequence":"additional","affiliation":[{"name":"Element Iowa City (JMI Laboratories) North Liberty Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2025.07.081"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","unstructured":"B. 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