{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T11:00:06Z","timestamp":1785236406209,"version":"3.55.0"},"reference-count":53,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Accurate species\u2010level diagnosis of dermatophytes, particularly\n                      <jats:italic>Trichophyton rubrum<\/jats:italic>\n                      , is important for guiding targeted antifungal therapy and improving the management of recurrent or atypical superficial mycoses. However, routine microscopy has only moderate sensitivity and cannot resolve fungal species; fungal culture is time\u2010consuming, and real\u2010time quantitative PCR (qPCR) requires costly instrumentation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To develop and clinically evaluate CRI\u2010RUB, a CRISPR\u2013Cas12a\u2013based assay integrating recombinase\u2010aided amplification (RAA) and a quantum\u2010dot (QD) fluorescent lateral\u2010flow strip for rapid species\u2010level detection of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>T. rubrum<\/jats:italic>\n                      <\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients\/Methods<\/jats:title>\n                    <jats:p>Analytical sensitivity and specificity were assessed using plasmid standards and a panel of common cutaneous fungi. A total of 140 clinical specimens were tested by both CRI\u2010RUB and TaqMan qPCR. Discrepant or grey\u2010zone qPCR results (Ct 33\u201334), together with a subset of concordant cases, were further arbitrated by internal transcribed spacer (ITS) sequencing to establish a composite reference standard.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      CRI\u2010RUB achieved a visual limit of detection of 2.3\u2009\u00d7\u200910\n                      <jats:sup>1<\/jats:sup>\n                      copies\/\u03bcL and showed no cross\u2010reactivity with other common cutaneous fungi. Among the 140 clinical specimens, CRI\u2010RUB showed a sensitivity of 96.1% (73\/76), specificity of 100.0% (64\/64), positive predictive value of 100.0% (73\/73), negative predictive value of 95.5% (64\/67) and Cohen's \u03ba of 0.96 compared with the composite reference standard, indicating excellent concordance. The complete workflow required approximately 85\u2009min.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      CRI\u2010RUB provides a rapid, sensitive, and specific approach for species\u2010level detection of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>T. rubrum<\/jats:italic>\n                      <\/jats:styled-content>\n                      . By combining CRISPR\u2013Cas12a detection with QD\u2010based lateral\u2010flow readout, this assay shows potential for near\u2010patient or point\u2010of\u2010care dermatophyte diagnosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.70208","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T09:13:29Z","timestamp":1785230009000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A CRISPR\u2013Cas12a and Quantum\u2010Dot Lateral\u2010Flow Assay for Rapid Species\u2010Level Detection of\n                    <i>Trichophyton rubrum<\/i>"],"prefix":"10.1111","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5151-7506","authenticated-orcid":false,"given":"Xiaoping","family":"Hu","sequence":"first","affiliation":[{"name":"Shanghai Skin Disease Clinical College The Fifth Clinical Medical College of Anhui Medical University, Shanghai Skin Disease Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Xu","sequence":"additional","affiliation":[{"name":"Shanghai Skin Disease Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyuan","family":"Shen","sequence":"additional","affiliation":[{"name":"Shanghai Skin Disease Clinical College The Fifth Clinical Medical College of Anhui Medical University, Shanghai Skin Disease Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Chai","sequence":"additional","affiliation":[{"name":"The Center for Basic Research and Innovation of Medicine and Pharmacy (MOE), School of Pharmacy Naval Medical University  Shanghai China"},{"name":"Department of Organic Chemistry, School of Pharmacy Naval Medical University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwen","family":"Tan","sequence":"additional","affiliation":[{"name":"Shanghai Skin Disease Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuepeng","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Medicine Shanghai University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianjuan","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai Skin Disease Hospital, School of Medicine Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qixia","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of General Practice Xi'an Central Hospital  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4953-0226","authenticated-orcid":false,"given":"Fei","family":"Tan","sequence":"additional","affiliation":[{"name":"Shanghai Skin Disease Clinical College The Fifth Clinical Medical College of Anhui Medical University, Shanghai Skin Disease Hospital  Shanghai China"},{"name":"Shanghai Skin Disease Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"The Center for Basic Research and Innovation of Medicine and Pharmacy (MOE), School of Pharmacy Naval Medical University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,28]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100507.2008.01606.x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof9060669"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525\u20101470.2012.01866.x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2010.02.048"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012258"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics14050472"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof8050464"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00894\u201024"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41378\u2010025\u201001057\u20104"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41378\u2010025\u201000977\u20105"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2024.127327"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar6245"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2023.125413"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551\u2010023\u201001033\u20101"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010025\u201004711\u20101"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2024.116740"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010020\u20100513\u20104"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c03883"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios13080786"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2016.11.042"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2021.130139"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28227496"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2024.112117"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010023\u201012509\u20100"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551\u2010021\u201000760\u20107"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof8080831"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid3009.240118"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1155\/drp\/1347872"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/23144599.2020.1850204"},{"issue":"11","key":"e_1_2_11_31_1","first-page":"703","article-title":"Rapid Extraction of Genomic DNA From Filamentous Fungi by Microwave Method","volume":"33","author":"Tian Y. Q.","year":"2008","journal-title":"Chinese Journal of Antibiotics"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1117018109"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201013\u2010134"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604\u2010020\u201004578\u2010z"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ynexs.2025.100059"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879\u2010017\u20102258\u20103"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1071\/MA22005"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.14198"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7110949"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2024.11721"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1017\/ash.2021.201"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.11.3.415"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S431526"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00903\u201023"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios12080636"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xops.2024.100522"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112445"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113005"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12092158"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myu088"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046\u2010016\u20100073\u20109"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43856\u2010025\u201000933\u20104"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2022.132767"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf5573"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.70208","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/myc.70208","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.70208","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T10:15:01Z","timestamp":1785233701000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.70208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":53,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/myc.70208"],"URL":"https:\/\/doi.org\/10.1111\/myc.70208","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2025-12-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70208"}}