{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:58:17Z","timestamp":1790405897665,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007378","name":"Universidade Federal de Juiz de Fora","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007378","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ\u201000995\u201025"],"award-info":[{"award-number":["APQ\u201000995\u201025"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Opportunistic yeasts account for a substantial proportion of invasive fungal infections and are important agents of healthcare\u2010associated infections, especially in intensive care units (ICUs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To describe the clinical and epidemiological characteristics of hospitalized patients with positive cultures for medically relevant yeasts and correlate these data with antifungal susceptibility, biocide tolerance and phenotypic efflux pump activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Ninety\u2010four clinical isolates were analysed: 31\n                      <jats:italic>Trichosporon asahii<\/jats:italic>\n                      , 39\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Candida albicans<\/jats:italic>\n                      <\/jats:styled-content>\n                      and 24 other yeasts. Antifungal susceptibility and biocide tolerance were assessed by disk diffusion, and phenotypic efflux pump activity was evaluated using ethidium bromide. Clinical and epidemiological data were retrieved from electronic health records.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Most isolates (73.4%) originated from ICUs. Urine (55.3%) was the most frequent clinical specimen. Overall mortality was 62.7%, reaching 71.8% among patients with\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C.\u2009albicans<\/jats:italic>\n                      <\/jats:styled-content>\n                      . Notably, 68.1% of patients did not receive antifungal therapy after diagnosis. All\n                      <jats:italic>T.\u2009asahii<\/jats:italic>\n                      isolates were susceptible to azoles and resistant to caspofungin.\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C.\u2009albicans<\/jats:italic>\n                      <\/jats:styled-content>\n                      showed high susceptibility to amphotericin B (100%) and caspofungin (93%). Phenotypic efflux pump activity was observed in all evaluated yeasts. Sodium hypochlorite showed greater antifungal activity, with inhibition zones of up to 18.0\u2009mm, while hydrogen peroxide (4.25%) and benzalkonium chloride (5%) showed minimal zones (~6\u2009mm) for all isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Opportunistic yeast infections continue to pose a major challenge in healthcare settings. Our findings highlight the critical importance of accurate laboratory identification, ongoing surveillance of antifungal susceptibility, and effective infection prevention and control strategies, including the rational selection of biocides, to improve patient outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.70216","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:53:38Z","timestamp":1788522818000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antifungal Susceptibility Profile and Biocide Tolerance in Clinically Relevant Yeasts: Implications for Therapy and Hospital Infection Control"],"prefix":"10.1111","volume":"69","author":[{"given":"Yasmim Passos","family":"Lima","sequence":"first","affiliation":[{"name":"Federal University of Juiz de Fora \u2013 UFJF  Juiz de Fora MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia Rebello","family":"Machado","sequence":"additional","affiliation":[{"name":"Federal University of Juiz de Fora \u2013 UFJF  Juiz de Fora MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Villela","family":"Bastos","sequence":"additional","affiliation":[{"name":"Cortes Villela Laboratory  Juiz de Fora MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor Quinet de Andrade","family":"Bastos","sequence":"additional","affiliation":[{"name":"Cortes Villela Laboratory  Juiz de Fora MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Quinet de Andrade","family":"Bastos","sequence":"additional","affiliation":[{"name":"Cortes Villela Laboratory  Juiz de Fora MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 Netto","family":"Bastos","sequence":"additional","affiliation":[{"name":"Department of Morphology Federal University of Juiz de Fora \u2013 UFJF  Juiz de Fora MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio Galuppo","family":"Diniz","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Microbiology, and Immunology Federal University of Juiz de Fora \u2013 UFJF  Juiz de Fora MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vania L\u00facia","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Microbiology, and Immunology Federal University of Juiz de Fora \u2013 UFJF  Juiz de Fora MG Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7681-3268","authenticated-orcid":false,"given":"Vanessa Cordeiro","family":"Dias","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Microbiology, and 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