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Antifungal susceptibility testing,\n                      <jats:italic>CYP51A<\/jats:italic>\n                      amplification and sequencing and expression analysis of\n                      <jats:italic>CYP51A<\/jats:italic>\n                      and\n                      <jats:italic>ABC1<\/jats:italic>\n                      under voriconazole exposure were performed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Antifungal susceptibility testing showed broadly elevated azole MICs, with the FSSC displaying the least favourable profile. Comparative CYP51A analysis revealed distinct amino acid signatures among the three species complexes, including residues at positions equivalent to canonical azole resistance hotspots in\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                      <\/jats:styled-content>\n                      . However, no simple relationship was observed between CYP51A amino acid profiles and azole MIC values. In contrast, voriconazole exposure induced marked\n                      <jats:italic>CYP51A<\/jats:italic>\n                      upregulation in all tested isolates, with substantially higher expression in the higher\u2010MIC FSSC isolate.\n                      <jats:italic>ABC1<\/jats:italic>\n                      was also upregulated in FSSC isolates, although at markedly lower levels than\n                      <jats:italic>CYP51A<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These findings support a multifactorial model of azole response in\n                      <jats:italic>Fusarium<\/jats:italic>\n                      species complex strains, involving conserved CYP51A sequence features, strong target\u2010gene induction under antifungal stress, and a lower but detectable contribution of efflux\u2010associated mechanisms.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.70220","type":"journal-article","created":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T08:11:51Z","timestamp":1788595911000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Multifactorial Azole Response in Clinically Relevant\n                    <i>Fusarium<\/i>\n                    Species Complexes"],"prefix":"10.1111","volume":"69","author":[{"given":"Patr\u00edcia Helena","family":"Grizante Bari\u00e3o","sequence":"first","affiliation":[{"name":"Department of Clinical Analyses, Toxicology, and Food Science, School of Pharmaceutical Sciences of Ribeirao Preto University of Sao Paulo  Ribeirao Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludmilla","family":"Tonani","sequence":"additional","affiliation":[{"name":"Department of Clinical 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