{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T15:24:21Z","timestamp":1787585061921,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,1,21]],"date-time":"2014-01-21T00:00:00Z","timestamp":1390262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropathology Appl Neurobio"],"published-print":{"date-parts":[[2014,2]]},"abstract":"Aims<\/jats:title>Synaptic vesicle proteins 2 (SV<\/jats:styled-content>2) are neuronal vesicles membrane glycoproteins that appear as important targets in the treatment of partial and generalized epilepsies. Therefore, we analysed the expression of SV<\/jats:styled-content>2 isoforms in the hippocampus of patients with temporal lobe epilepsy (TLE<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>SV<\/jats:styled-content>2A<\/jats:styled-content>, SV<\/jats:styled-content>2B<\/jats:styled-content> and SV<\/jats:styled-content>2C<\/jats:styled-content> immunostaining and Q<\/jats:styled-content>uantiG<\/jats:styled-content>ene branched DNA<\/jats:styled-content> assay were performed on biopsies from 31 consecutive TLE<\/jats:styled-content> patients with mesial temporal sclerosis (MTS<\/jats:styled-content>) and compared with 10 autopsy controls. SV<\/jats:styled-content>2 expression was further compared with T<\/jats:styled-content>imm's staining, and synaptophysin, Z<\/jats:styled-content>inc transporter 3 (ZnT<\/jats:styled-content>3), dynorphin, vesicular glutamate transporter 1 (VGLUT<\/jats:styled-content>1) and vesicular GABA<\/jats:styled-content> transporter (VGAT<\/jats:styled-content>) expression.<\/jats:p><\/jats:sec>Results<\/jats:title>In TLE<\/jats:styled-content> patients, SV<\/jats:styled-content>2A<\/jats:styled-content> and SV<\/jats:styled-content>2B<\/jats:styled-content> expression was decreased in areas of synaptic loss. SV<\/jats:styled-content>2C<\/jats:styled-content>, which is weakly expressed or absent in the hippocampus of controls, was overexpressed in 10\/11 cases with classical MTS<\/jats:styled-content>1A<\/jats:styled-content> and mossy fibre sprouting but not in cases with other types of MTS<\/jats:styled-content>. SV<\/jats:styled-content>2C<\/jats:styled-content> staining was located in the inner molecular layer of the dentate gyrus and colocalized with dynorphin, ZnT<\/jats:styled-content>3 and VGLUT<\/jats:styled-content>1, suggesting selective expression in presynaptic glutamatergic Zn<\/jats:styled-content>2+<\/jats:sup>\u2010rich terminals of abnormal sprouting fibres. SV<\/jats:styled-content>2 expression patterns correlated with histological subtypes of MTS<\/jats:styled-content>, but not with clinical features or therapeutic regimens in this patient cohort.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>In classical MTS<\/jats:styled-content>1A<\/jats:styled-content>, the expression of SV<\/jats:styled-content>2 isoforms is altered with a marked decrease of SV<\/jats:styled-content>2A<\/jats:styled-content> and SV<\/jats:styled-content>2B<\/jats:styled-content> paralleling synaptic loss and a selective increase of SV<\/jats:styled-content>2C<\/jats:styled-content> in sprouting mossy fibres. These findings suggest a different physiology of sprouting synapses and the possibility to target them with SV<\/jats:styled-content>2C<\/jats:styled-content>\u2010specific strategies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nan.12054","type":"journal-article","created":{"date-parts":[[2013,4,26]],"date-time":"2013-04-26T10:54:40Z","timestamp":1366973680000},"page":"191-204","source":"Crossref","is-referenced-by-count":63,"title":["Expression pattern of synaptic vesicle protein 2 (SV<\/scp>2) isoforms in patients with temporal lobe epilepsy and hippocampal sclerosis"],"prefix":"10.1111","volume":"40","author":[{"given":"J.","family":"Cr\u00e8vec\u0153ur","sequence":"first","affiliation":[{"name":"Laboratory of Neuropathology GIGA\u2010Neurosciences University of Liege Liege Belgium"},{"name":"Laboratory of Developmental Neurobiology GIGA\u2010Neurosciences University of Liege Liege Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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