{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:11:23Z","timestamp":1762161083684},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T00:00:00Z","timestamp":1431475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropathology Appl Neurobio"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>The serotonin 2<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> receptor (<jats:styled-content style=\"fixed-case\">HTR2A<\/jats:styled-content>) is widely expressed in the brain and involved in the modulation of fear, mood, anxiety and other symptoms. <jats:styled-content style=\"fixed-case\">HTR2A<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>HTR2A<\/jats:italic><\/jats:styled-content> gene variations are implicated in depression, schizophrenia, anxiety and obsessive\u2010compulsive disorder. To understand <jats:styled-content style=\"fixed-case\">HTR2A<\/jats:styled-content> signalling changes in psychiatric or neurodegenerative disorders, its normal pattern of brain expression and region specificity during development and ageing needs to be clarified. The aim of the present study was to assess <jats:styled-content style=\"fixed-case\"><jats:italic>HTR2A<\/jats:italic><\/jats:styled-content> expression through developmental and ageing stages in six brain regions in <jats:italic>post mortem<\/jats:italic> human brain samples from individuals with no clinical or neuropathological evidence of neuropsychiatric disorders and to investigate the interaction with the rs6311 <jats:styled-content style=\"fixed-case\"><jats:italic>HTR2A<\/jats:italic><\/jats:styled-content> promoter polymorphism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and protein were isolated from <jats:italic>post mortem<\/jats:italic> brain samples including six regions (frontal cortex, striatum, amygdala, thalamus, brain stem and cerebellum) from 55 individuals. <jats:styled-content style=\"fixed-case\"><jats:italic>HTR2A<\/jats:italic> mRNA<\/jats:styled-content> levels were assessed using quantitative real\u2010time <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HTR2A<\/jats:styled-content> protein levels \u2013 with Western blot. The rs6311 <jats:styled-content style=\"fixed-case\"><jats:italic>HTR2A<\/jats:italic><\/jats:styled-content> polymorphism was analysed with genotyping.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that <jats:styled-content style=\"fixed-case\"><jats:italic>HTR2A<\/jats:italic> mRNA<\/jats:styled-content> and protein levels are differentially regulated with age in different brain regions studied, but are not affected by gender. Significant changes in <jats:styled-content style=\"fixed-case\">HTR2A<\/jats:styled-content> expression with age were found in frontal cortex, amygdala, thalamus, brain stem and cerebellum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results show plasticity and region specificity of <jats:styled-content style=\"fixed-case\">HTR2A<\/jats:styled-content> expression regulation in human brain with age, which may be important for the interaction with other neurotransmitter systems and for the occurrence of developmental periods with increased vulnerability to neuropsychiatric or neurodegenerative disorders.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nan.12167","type":"journal-article","created":{"date-parts":[[2014,7,8]],"date-time":"2014-07-08T07:44:46Z","timestamp":1404805486000},"page":"520-532","source":"Crossref","is-referenced-by-count":13,"title":["Region\u2010specific regulation of the serotonin 2<scp>A<\/scp> receptor expression in development and 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