{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T18:27:03Z","timestamp":1787596023371,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,4,30]],"date-time":"2015-04-30T00:00:00Z","timestamp":1430352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Medical Faculty of the Johannes Gutenberg University Mainz"},{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropathology Appl Neurobio"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>The antiphospholipid syndrome (<jats:styled-content style=\"fixed-case\">APS<\/jats:styled-content>) is an autoimmune disease characterized by high titres of auto\u2010antibodies (<jats:styled-content style=\"fixed-case\">aPL<\/jats:styled-content>) leading to thrombosis and consequent infarcts. However, many affected patients develop neurological symptoms in the absence of stroke. Similarly, in a mouse model of this disease (<jats:styled-content style=\"fixed-case\">eAPS<\/jats:styled-content>), animals consistently develop behavioural abnormalities despite lack of ischemic brain injury. Therefore, the present study was designed to identify structural alterations of hippocampal neurones underlying the neurological symptoms in <jats:styled-content style=\"fixed-case\">eAPS<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Adult female <jats:styled-content style=\"fixed-case\">Balb\/C<\/jats:styled-content> mice were subjected to either induction of <jats:styled-content style=\"fixed-case\">eAPS<\/jats:styled-content> by immunization with \u03b22\u2010Glycoprotein 1 or to a control group. After sixteen weeks animals underwent behavioural and cognitive testing using Staircase test (experiment 1 and 2) and <jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>\u2010maze alternation test (experiment 1) and were tested for serum <jats:styled-content style=\"fixed-case\">aPL<\/jats:styled-content> levels (both experiments). Animals of experiment 1 (<jats:italic>n<\/jats:italic>\u2009=\u20097\/group) were used for hippocampal neurone analysis using <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>olgi\u2010<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>ox staining. Animals of experiment 2 (<jats:italic>n<\/jats:italic>\u2009=\u20097\/group) were used to analyse molecular markers of total dendritic integrity (<jats:styled-content style=\"fixed-case\">MAP<\/jats:styled-content>2), presynaptic plasticity (synaptobrevin 2\/<jats:styled-content style=\"fixed-case\">VAMP<\/jats:styled-content>2) and dendritic spines (synaptopodin) using immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">eAPS<\/jats:styled-content> mice developed increased <jats:styled-content style=\"fixed-case\">aPL<\/jats:styled-content> titres and presented with abnormal behaviour and impaired short term memory. Further, they revealed a reduction of dendritic complexity of hippocampal <jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>1 neurones as reflected by decreased dendritic length, arborization and spine density, respectively. Additional decrease of the spine\u2010associated protein expression of Synaptopodin points to dendritic spines as major targets in the pathological process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Reduction of hippocampal dendritic complexity may represent the structural basis for the behavioural and cognitive abnormalities of <jats:styled-content style=\"fixed-case\">eAPS<\/jats:styled-content> mice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nan.12180","type":"journal-article","created":{"date-parts":[[2014,9,9]],"date-time":"2014-09-09T08:14:37Z","timestamp":1410250477000},"page":"657-671","source":"Crossref","is-referenced-by-count":23,"title":["Mice with experimental antiphospholipid syndrome display hippocampal dysfunction and a reduction of dendritic complexity in hippocampal <scp>CA1<\/scp> neurones"],"prefix":"10.1111","volume":"41","author":[{"given":"Katrin","family":"Frauenknecht","sequence":"first","affiliation":[{"name":"Department of Neuropathology University Medical Center of the Johannes Gutenberg University Mainz  Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aviva","family":"Katzav","sequence":"additional","affiliation":[{"name":"Department of Neurology Chaim Sheba Medical Center Sackler Faculty of Medicine Tel Aviv University  Tel Hashomer Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronen","family":"Weiss Lavi","sequence":"additional","affiliation":[{"name":"Department of Neurology Chaim Sheba Medical Center Sackler Faculty of Medicine Tel Aviv University  Tel Hashomer Israel"},{"name":"Sagol School of Neuroscience Tel Aviv University  Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avishag","family":"Sabag","sequence":"additional","affiliation":[{"name":"Department of Neurology Chaim Sheba Medical Center Sackler Faculty of Medicine Tel Aviv University  Tel Hashomer Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Otten","sequence":"additional","affiliation":[{"name":"Department of Neuropathology University Medical Center of the Johannes Gutenberg University Mainz  Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joab","family":"Chapman","sequence":"additional","affiliation":[{"name":"Department of Neurology Chaim Sheba Medical Center Sackler Faculty of Medicine Tel Aviv University  Tel Hashomer Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clemens J.","family":"Sommer","sequence":"additional","affiliation":[{"name":"Department of Neuropathology University Medical Center of the Johannes Gutenberg University Mainz  Mainz Germany"},{"name":"Focus Program Translational Neurosciences (FTN) Rhine Main Neuroscience Network (rmn<sub>2<\/sub>)  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,4,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1112830"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2006.01753.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.26.5.749"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1191\/0961203302lu255oa"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2012.06.011"},{"key":"e_1_2_7_7_1","first-page":"404815","article-title":"Significant changes in the levels of secreted cytokines in brains of experimental antiphospholipid syndrome mice","volume":"2012","author":"Menachem A","year":"2012","journal-title":"Autoimmune Dis"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2013.11.006"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-7634(01)00041-0"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2005.04.005"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddr457"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2012.04.008"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115749"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00432023"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002130050110"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(92)91352-F"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(97)00163-5"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/171387a0"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2010.05.043"},{"key":"e_1_2_7_20_1","volume-title":"The Mouse Brain in Stereotaxic Coordinates, 2nd edn","author":"Paxinos G","year":"2001"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-11-92"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(00)00050-6"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.20664"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.20554"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2011.11.053"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0173(90)90013-E"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(89)90383-7"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1994.63031012.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/362318a0"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1078\/0171-9335-00196"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2007.10.012"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(20000306)418:2<164::AID-CNE4>3.0.CO;2-0"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1832384100"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5528-08.2009"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2007.12.004"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(98)00027-6"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1191\/096120399678847524"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8749.2011.03922.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1191\/0961203303lu409oa"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psyneuen.2007.09.004"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2013.05.011"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.npp.1300446"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-03-00854.2002"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-11-04530.2002"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2009.02.020"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1080\/10446670410001670526"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-08-2349"}],"container-title":["Neuropathology and Applied Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnan.12180","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nan.12180","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T13:15:10Z","timestamp":1695561310000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nan.12180"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,30]]},"references-count":46,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1111\/nan.12180"],"URL":"https:\/\/doi.org\/10.1111\/nan.12180","archive":["Portico"],"relation":{},"ISSN":["0305-1846","1365-2990"],"issn-type":[{"value":"0305-1846","type":"print"},{"value":"1365-2990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,30]]}}}