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Prion\u2010like transmission of these aggregates between cells has been demonstrated. The cell\u2010to\u2010cell transmission mechanisms of these protein aggregates and the susceptibility of different kinds of neuronal cells to these toxic assemblies still need assessment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Here, we documented the binding to and internalization by differentiated and undifferentiated neuroblastoma cells of exogenous fibrillar <jats:styled-content style=\"fixed-case\">HTTE<\/jats:styled-content>xon1 and polyglutamine (poly<jats:styled-content style=\"fixed-case\">Q<\/jats:styled-content>) polypeptides containing the same number of glutamines. We assessed the contribution of endocytosis to fibrillar <jats:styled-content style=\"fixed-case\">HTTE<\/jats:styled-content>xon1 uptake, their intracellular localization and fate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed that undifferentiated neuroblastoma cells were more susceptible to fibrillar <jats:styled-content style=\"fixed-case\">HTTE<\/jats:styled-content>xon1 and poly<jats:styled-content style=\"fixed-case\">Q<\/jats:styled-content> than their differentiated counterparts. Furthermore, we demonstrated that exogenous <jats:styled-content style=\"fixed-case\">HTTE<\/jats:styled-content>xon1 aggregates are mainly taken up by endocytosis and directed to lysosomal compartments in both mitotic and quiescent cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These data suggest that the rates of endocytic processes that differ in mitotic and quiescent cells strongly impact the uptake of exogenous <jats:styled-content style=\"fixed-case\">HTTE<\/jats:styled-content>xon1 and poly<jats:styled-content style=\"fixed-case\">Q<\/jats:styled-content> fibrils. This may be either the consequence of distinct metabolisms or distributions of specific protein partners for amyloid\u2010like assemblies at the surface of highly dividing versus quiescent cells. Our results highlight the importance of endocytic processes in the internalization of exogenous <jats:styled-content style=\"fixed-case\">HTTE<\/jats:styled-content>xon1 fibrils and suggest that a proportion of those assemblies reach the cytosol where they can amplify by recruiting the endogenous protein after escaping, by yet an unknown process, from the endo\u2010lysosomal compartments.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nan.12258","type":"journal-article","created":{"date-parts":[[2015,6,26]],"date-time":"2015-06-26T04:18:49Z","timestamp":1435292329000},"page":"137-152","source":"Crossref","is-referenced-by-count":17,"title":["Binding, internalization and fate of <scp>H<\/scp>untingtin <scp>E<\/scp>xon1 fibrillar assemblies in mitotic and nonmitotic neuroblastoma cells"],"prefix":"10.1111","volume":"42","author":[{"given":"G.","family":"Ruiz\u2010Arlandis","sequence":"first","affiliation":[{"name":"Paris\u2010Saclay Institute of Neuroscience CNRS  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Pieri","sequence":"additional","affiliation":[{"name":"Paris\u2010Saclay Institute of Neuroscience CNRS  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Bousset","sequence":"additional","affiliation":[{"name":"Paris\u2010Saclay Institute of Neuroscience CNRS  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Melki","sequence":"additional","affiliation":[{"name":"Paris\u2010Saclay Institute of Neuroscience CNRS  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5334.1990"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4850"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1016\/0092-8674(93)90585-E","article-title":"A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes","volume":"72","author":"HD Collaborative Research Group","year":"1993","journal-title":"Cell"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.210395797"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13304-1"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00041.2009"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.8.4604"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.603332"},{"key":"e_1_2_8_10_1","first-page":"109","article-title":"On chorea","volume":"26","author":"Huntington G","year":"1872","journal-title":"Med Surg Rep"},{"key":"e_1_2_8_11_1","unstructured":"ParkinsonJ.An essay on the shaking palsy. 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