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However, little is known about the intrinsic regulatory machinery underlying the self\u2010renewing division of NPCs in the brain after stroke.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Polycomb repressor complex 1\u2010chromobox7 (CBX7) has emerged as a key regulator in several cellular processes including stem cell self\u2010renewal and cancer cell proliferation. The hypoxic environment triggering NPC self\u2010renewal after CBX7 activation remains unknown. In this study, we found that the upregulation of CBX7 during hypoxia and ischaemia appeared to be from hypoxia\u2010inducible factor\u20101\u03b1 (HIF\u20101\u03b1) activation. During hypoxia, the HIF\u20101\u03b1\u2013CBX7 cascade modulated NPC proliferation <jats:italic>in vitro<\/jats:italic>. NPC numbers significantly decreased in CBX7 knockout mice generated using CRISPR\/Cas9 genome editing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We provided the novel insight that CBX7 expression is regulated through HIF\u20101\u03b1 activation, which plays an intrinsically modulating role in NPC proliferation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nan.12585","type":"journal-article","created":{"date-parts":[[2019,10,20]],"date-time":"2019-10-20T00:48:37Z","timestamp":1571532517000},"page":"391-405","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Mechanisms of ischaemic neural progenitor proliferation: a regulatory role of the HIF\u20101\u03b1\u2010CBX7 pathway"],"prefix":"10.1111","volume":"46","author":[{"given":"H.\u2010Y.","family":"Chiu","sequence":"first","affiliation":[{"name":"Children\u2019s Hospital China Medical University and Hospital Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.\u2010T.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Taichung Veterans General Hospital Taichung Taiwan"},{"name":"Graduate Institute of Medical Sciences National Defense Medical Center Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.\u2010H.","family":"Lee","sequence":"additional","affiliation":[{"name":"Eshelman School of Pharmacy University of North Carolina Chapel Hill NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Zhao","sequence":"additional","affiliation":[{"name":"Eshelman School of Pharmacy University of North Carolina Chapel Hill NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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