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YTHDF1 and YTHDF3 showed opposing expression patterns in the cerebellum and the frontal and cingulate cortices. Machine learning quantitative image analysis revealed that m<jats:sup>6<\/jats:sup>A\u2010modified transcripts were significantly altered in localisation and abundance in disease tissue with significant decreases in m<jats:sup>6<\/jats:sup>A\u2010RNAs in Parkinson's disease, and significant increases in m<jats:sup>6<\/jats:sup>A\u2010RNA abundance in dementia with Lewy bodies. MCI tissue showed variability across regions but similar to DLB; in brain areas with an overall significant increase in m<jats:sup>6<\/jats:sup>A\u2010RNAs, modified RNAs within dendritic processes were reduced. Using mass spectrometry proteomic datasets to corroborate our findings, we found significant changes in YTHDF3 and m<jats:sup>6<\/jats:sup>A anti\u2010reader protein abundance in Alzheimer's disease (AD) and asymptomatic AD\/MCI tissue and correlation with cognitive resilience.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results provide evidence for disrupted m<jats:sup>6<\/jats:sup>A regulation in Lewy body diseases and a plausible mechanism through which RNA processing could contribute to the formation of Lewy bodies and other dementia\u2010associated pathological aggregates. The findings suggest that manipulation of epitranscriptomic processes influencing translational control may lead to new therapeutic approaches for neurodegenerative diseases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nan.12885","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T01:11:02Z","timestamp":1675041062000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["m<sup>6<\/sup>A mRNA methylation in human brain is disrupted in Lewy body disorders"],"prefix":"10.1111","volume":"49","author":[{"given":"Braulio","family":"Martinez De La Cruz","sequence":"first","affiliation":[{"name":"Division of Cells, Organisms and Molecular Genetics, School of Life Sciences University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Gell","sequence":"additional","affiliation":[{"name":"School of Life Sciences Imaging Facility University of 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