{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T18:54:03Z","timestamp":1785783243854,"version":"3.56.0"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T00:00:00Z","timestamp":1688428800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017237","name":"Hans-Gabriel och Alice Trolle-Wachtmeisters stiftelse f\u00f6r medicinsk forskning","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017237","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005423","name":"Akademiska Sjukhuset","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005423","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neuropathology Appl Neurobio"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study aims to study the association between pancreatic islet amyloid polypeptide (IAPP) and Alzheimer's disease neuropathological change (ADNC) in brain biopsies obtained from subjects with idiopathic normal pressure hydrocephalus (iNPH) and in post\u2010mortem (PM) brain samples obtained from aged individuals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>For the immunohistochemical (IHC) analyses, two IAPP antibodies (Abs), monoclonal and polyclonal, and Abs directed towards ADNC were applied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The iNPH cohort included 113 subjects. Amyloid\u2010\u03b2 (A\u03b2) was detected in 50% and hyperphosphorylated \u03c4 (HP\u03c4) in 47% of the cases. Concomitant pathology was seen in 32%. The PM cohort included 77 subjects. A\u03b2 was detected in 69% and HP\u03c4 in 91% of the cases. Combined A\u03b2\/HP\u03c4 pathology was seen in 62%. Reactivity for the monoclonal IAPP was not detected in the brain tissue in either of the cohorts. Reactivity for the polyclonal IAPP was observed in all 77\u2009PM brain samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>There was no specific expression of IAPP in human brain tissue; hence, an association between IAPP and ADNC is not assessable. Of note, the observed reactivity of the polyclonal IAPP Ab was not reproduced with a specific monoclonal Ab; thus, we considered the observed staining with the polyclonal Ab to be unreliable. When using IHC, several pitfalls, especially the choice of an Ab, always need to be considered. Polyclonal Abs cross\u2010react with other epitopes and proteins, thus leading to false\u2010positive results. This seems to be the case for the polyclonal IAPP Abs in the human brain.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nan.12917","type":"journal-article","created":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T02:29:00Z","timestamp":1686796140000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Is islet amyloid polypeptide indeed expressed in the human brain?"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1043-5385","authenticated-orcid":false,"given":"Sylwia","family":"Libard","sequence":"first","affiliation":[{"name":"Department of Immunology, Genetics and Pathology Uppsala University  Uppsala Sweden"},{"name":"Department of Pathology Uppsala University Hospital  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Alafuzoff","sequence":"additional","affiliation":[{"name":"Department of Pathology Uppsala 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