{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:53:11Z","timestamp":1772160791391,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T00:00:00Z","timestamp":1705449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006146","name":"F\u00f6rdergemeinschaft Kinderkrebs-Zentrum Hamburg","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006146","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005972","name":"Deutsche Krebshilfe","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005972","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neuropathology Appl Neurobio"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Pilocytic astrocytomas (PA) in adults are rare and may be challenging to identify based only on histomorphology. Compared to their paediatric counterparts, they are reportedly molecularly more diverse and associated with a worse prognosis. We aimed to describe the characteristics of adult PAs more precisely by comprehensively profiling a series of 79 histologically diagnosed adult cases (\u226518\u2009years).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We performed global DNA methylation profiling and DNA and RNA panel sequencing and integrated the results with clinical data. We further compared the molecular characteristics of adult and paediatric PAs that had a significant match to one of the established PA methylation classes in the Heidelberg brain tumour classifier.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The mean age in our cohort was 33\u2009years, and 43% of the tumours were located supratentorially. Based on methylation profiling, only 39% of the cases received a significant match to a PA methylation class. Sixteen per cent matched a different tumour type, and 45% had a Heidelberg classifier score &lt;0.9 with an affiliation to diverse established methylation classes in\n                      <jats:italic>t<\/jats:italic>\n                      \u2010SNE analyses. Although the\n                      <jats:italic>KIAA1549::BRAF<\/jats:italic>\n                      fusion was found in 98% of paediatric PAs, this was true for only 27% of histologically defined and 55% of adult PAs defined by methylation profiling.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      A particularly high fraction of adult tumours with histological features of PA do not match current PA methylation classes, indicating ambiguous histology and an urgent need for molecular profiling. Moreover, even in adult PAs with a match to a PA methylation class, the distribution of genetic drivers differs significantly from their paediatric counterparts (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01).\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nan.12949","type":"journal-article","created":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T06:38:00Z","timestamp":1702967880000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular refinement of pilocytic astrocytoma in adult patients"],"prefix":"10.1111","volume":"50","author":[{"given":"Helena","family":"Bode","sequence":"first","affiliation":[{"name":"Department of Pediatric Hematology and Oncology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"Research Institute Children's Cancer Center Hamburg  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catena","family":"Kresbach","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"Research Institute Children's Cancer Center Hamburg  Hamburg Germany"},{"name":"Institute of Neuropathology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"Mildred Scheel Cancer Career Center HaTriCS4 University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00f6rthe","family":"Holdhof","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"Research Institute Children's Cancer Center Hamburg  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario M.","family":"Dorostkar","sequence":"additional","affiliation":[{"name":"Center for Neuropathology Ludwig\u2010Maximilians\u2010University  Munich Germany"},{"name":"Karl Landsteiner Privatuniversit\u00e4t f\u00fcr Gesundheitswissenschaften  Saint P\u00f6lten Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick N.","family":"Harter","sequence":"additional","affiliation":[{"name":"Center for Neuropathology Ludwig\u2010Maximilians\u2010University  Munich Germany"},{"name":"Neurological Institute (Edinger Institute), University Hospital Frankfurt Goethe\u2010University  Frankfurt am Main Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Hench","sequence":"additional","affiliation":[{"name":"Division of Neuropathology, Institute of Medical Genetics and Pathology University of Basel  Basel 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