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Despite this critical role, pathologies in DG are less commonly described than those in the CA sectors in the diagnosis of mesial temporal lobe epilepsy (mTLE). To elucidate the role of the DG in mTLE, we analysed hippocampal sclerosis (HS), no\u2010HS, non\u2010TLE epilepsy control, and non\u2010epilepsy control cohorts using morphometry and gene expression profiling techniques. Morphometry techniques analysed DG cell spacing, nucleus size, and nucleus circularity. Our data show distinct DG morphometry and RNA expression profiles between HS and No\u2010HS. Dentate granule cells are more dispersed in patients with HS, and the DG shows an elevated expression of the complement system, apoptosis, and extracellular matrix remodelling\u2013related RNA. We also observe an overall decrease in neurogenesis\u2010related RNA in HS DG. Interestingly, regardless of the pathological diagnosis, the DG morphometry correlates with post\u2010operative outcomes. Increased cell spacing is observed in the DG of mTLE cases that achieve seizure freedom post\u2010operatively. This study reveals the possible prognostic value of DG morphometry, as well as supporting the notion that HS and no\u2010HS TLE may be distinct disease entities with differing contributing mechanisms.<\/jats:p>","DOI":"10.1111\/nan.13008","type":"journal-article","created":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T00:19:54Z","timestamp":1728346794000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Hippocampal dentate granule cells in temporal lobe epilepsy: A morphometry and transcriptomic study"],"prefix":"10.1111","volume":"50","author":[{"given":"Carolyn","family":"Twible","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Western University  London ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rober","family":"Abdo","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Western University  London ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chelsey","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Western University  London ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4928-2742","authenticated-orcid":false,"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Western University  London ON Canada"},{"name":"Department of Pathology and Laboratory Medicine London Health Sciences Centre  London ON Canada"},{"name":"Department of Clinical Neurological Sciences London Health Sciences Centre  London ON Canada"},{"name":"Department of Oncology London Health Sciences Centre  London ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,7]]},"reference":[{"key":"e_1_2_12_2_1","unstructured":"Epilepsy.World Health Organization."},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.pbr.2016.04.013"},{"key":"e_1_2_12_4_1","first-page":"1","article-title":"A comprehensive review of temporal lobe epilepsy","volume":"1","author":"Ladino LD","year":"2014","journal-title":"Neurol Dis"},{"key":"e_1_2_12_5_1","volume-title":"Mesial temporal lobe epilepsy","author":"Nayak C","year":"2023"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/630853"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.12220"},{"key":"e_1_2_12_8_1","volume-title":"The dentate gyrus: a comprehensive guide to structure, function, and clinical implications","author":"Scharfman H","year":"2007"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079\u20106123(07)63032\u20105"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP270056"},{"key":"e_1_2_12_11_1","first-page":"273","article-title":"The dentate gyrus as a regulated gate for the propagation of epileptiform activity","volume":"7","author":"Heinemann U","year":"1992","journal-title":"Epilepsy Res Suppl"},{"key":"e_1_2_12_12_1","first-page":"301","article-title":"The dentate gyrus as a control point for seizures in the hippocampus and beyond","volume":"7","author":"Lothman EW","year":"1992","journal-title":"Epilepsy Res Suppl"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/nan.12150"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750\u20103639.2012.00583.x"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006\u20108993(90)91601\u2010C"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0013\u20109580.2004.09004.x"},{"key":"e_1_2_12_17_1","first-page":"609","volume-title":"Surgical treatment of epilepsies","author":"Engel JJ","year":"1993"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yebeh.2021.107940"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201017204\u20105"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401\u2010009\u20100512\u20105"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.5414\/NP300509"},{"key":"e_1_2_12_22_1","unstructured":"NanoString.nCounter Analysis Systems Overview. 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