{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:51:34Z","timestamp":1758819094840},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,7,25]],"date-time":"2014-07-25T00:00:00Z","timestamp":1406246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Bio & Medical Technology Development Program","award":["2010-0019867 and 2012M3A9D12012054518"],"award-info":[{"award-number":["2010-0019867 and 2012M3A9D12012054518"]}]},{"name":"Mid-career Researcher Program","award":["2012-043084"],"award-info":[{"award-number":["2012-043084"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2014,8]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>Autosomal dominant polycystic kidney disease (ADPKD<\/jats:styled-content>) is a highly prevalent inherited disorder and results in the progressive development of cysts in both kidneys. In recent studies, several cytokines and growth factors secreted by the cyst\u2010lining epithelia were identified to be upregulated and promote cyst growth. According to our previous study, chemokines with a similar amino acid sequence as human interleukin\u20108 (IL<\/jats:styled-content>\u20108) are highly expressed in a rodent model with renal cysts. Therefore, in this study, we focused on whether IL<\/jats:styled-content>\u20108 signalling is associated with renal cyst formation, and tested the possibility of IL<\/jats:styled-content>\u20108 as a new therapeutic target for ADPKD<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>Expression of IL<\/jats:styled-content>\u20108 and its receptor were screened either by enzyme linked immunosorbent assay (ELISA<\/jats:styled-content>) or Western blot. Inhibited IL<\/jats:styled-content>\u20108 signalling by antagonist for IL<\/jats:styled-content>\u20108 receptor or gene silencing was tested in molecular levels, mainly through Western blot. And cell proliferation was measured by XTT<\/jats:styled-content> assays. Finally, a three\u2010dimensional culture was performed to understand how IL<\/jats:styled-content>\u20108 affected cyst formation, in vitro<\/jats:italic>.<\/jats:p><\/jats:sec>Results<\/jats:title>Interleukin\u20108 secretion and expression of its receptor highly increased in two different human ADPKD<\/jats:styled-content> cell lines (WT9<\/jats:styled-content>\u20107 and WT9<\/jats:styled-content>\u201012), compared to normal human renal cortical epithelial cell line. Cell proliferation, which is mediated by IL<\/jats:styled-content>\u20108 signal, was inhibited either by an antagonist or siRNA<\/jats:styled-content> targeting for IL<\/jats:styled-content>\u20108 receptor. Finally, a three\u2010dimensional culture showed an alleviation of cystogenesis in vitro<\/jats:italic>, after blocking the IL<\/jats:styled-content>\u20108 receptor signals.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>These results suggest that IL<\/jats:styled-content>\u20108 and its signalling molecules could be new biomarkers and a therapeutic target of ADPKD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nep.12261","type":"journal-article","created":{"date-parts":[[2014,4,14]],"date-time":"2014-04-14T02:46:53Z","timestamp":1397443613000},"page":"471-478","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Blockade of interleukin\u20108 receptor signalling inhibits cyst development in vitro<\/i>, via suppression of cell proliferation in autosomal polycystic kidney disease"],"prefix":"10.1111","volume":"19","author":[{"given":"Eun Ji","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Biological Science Sookmyung Women's University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seon Ah","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Biological Science Sookmyung Women's University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyo Won","family":"Mun","sequence":"additional","affiliation":[{"name":"Department of Biological Science Sookmyung Women's University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Hyun","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Biological Science Sookmyung Women's University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soo Young","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Institute of Bioscience and Biotechnology Hallym University Chuncheon Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun Young","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biological Science Sookmyung Women's University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong Hoon","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biological Science Sookmyung Women's University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.60.101707.125712"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.08.043"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1681\/ASN.V34984","article-title":"Cyst fluid from human autosomal dominant polycystic kidneys promotes cyst formation and expansion by renal epithelial cells in vitro","volume":"3","author":"Ye M","year":"1992","journal-title":"J. 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