{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T14:40:48Z","timestamp":1788446448497,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,8,25]],"date-time":"2014-08-25T00:00:00Z","timestamp":1408924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Sri Ramachandra University, Chennai"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2014,9]]},"abstract":"Abstract<\/jats:title>\n \n Aim<\/jats:title>\n \n Autosomal dominant polycystic kidney disease (\n ADPKD<\/jats:styled-content>\n ) is the most common hereditary and progressive renal disorder. It is also recognised as the most frequent genetic cause of chronic kidney diseases (\n CKD<\/jats:styled-content>\n ). In the present study, four tagging\n SNPs<\/jats:styled-content>\n and two more well studied polymorphisms (Intron 4\n VNTR<\/jats:styled-content>\n and\n Glu298Asp<\/jats:styled-content>\n ) the\n NOS3<\/jats:styled-content>\n gene were investigated to unravel the potential modifier effect of\n \n NOS3<\/jats:italic>\n <\/jats:styled-content>\n gene on the progression of\n CKD<\/jats:styled-content>\n in\n ADPKD<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n A total of 102\n ADPKD<\/jats:styled-content>\n patients and 106 controls were selected for the study. The\n tagSNPs<\/jats:styled-content>\n and\n Glu298Asp<\/jats:styled-content>\n polymorphisms were genotyped using\n FRET<\/jats:styled-content>\n \u2010based\n KASPar<\/jats:styled-content>\n method and intron\u20104\n VNTR<\/jats:styled-content>\n by polymerase chain reaction electrophoresis. The genotypes and haplotypes in the controls and\n ADPKD<\/jats:styled-content>\n subjects were analysed by \u03c7\n 2<\/jats:sup>\n tests and haploview software.\n M<\/jats:styled-content>\n antel\u2010\n H<\/jats:styled-content>\n aenszel stratified and univariate analyses were performed to estimate the influence of different genotypes between different\n CKD<\/jats:styled-content>\n stages and hypertension.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The\n tagSNPs<\/jats:styled-content>\n of\n NOS3<\/jats:styled-content>\n genotypes and haplotypes did not exhibit any significant differences between controls and\n ADPKD<\/jats:styled-content>\n patients. The significant linkage disequilibrium was observed between the rs3918184 and rs2853796 by forming\n LD<\/jats:styled-content>\n block. In univariate analysis, the age and family history of\n D<\/jats:styled-content>\n iabetes mellitus (\n DM<\/jats:styled-content>\n ) showed significant association with advancement of\n CKD<\/jats:styled-content>\n , but not with the\n eNOS<\/jats:styled-content>\n polymorphisms.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Our data suggests that there is no evidence for the involvement of\n NOS3<\/jats:styled-content>\n tag\n SNPs<\/jats:styled-content>\n in the progression to\n CKD<\/jats:styled-content>\n in\n ADPKD<\/jats:styled-content>\n patients. A systematic study using well validated functional\n SNPs<\/jats:styled-content>\n is necessary to clarify the role of the\n NOS3<\/jats:styled-content>\n gene in the development of\n CKD<\/jats:styled-content>\n in\n ADPKD<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/nep.12278","type":"journal-article","created":{"date-parts":[[2014,5,13]],"date-time":"2014-05-13T23:09:13Z","timestamp":1400022553000},"page":"537-541","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["NOS3 tagSNPs<\/scp>\n does not modify the chronic kidney disease progression in autosomal dominant polycystic kidney disease"],"prefix":"10.1111","volume":"19","author":[{"given":"Gnanasambandan","family":"Ramanathan","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences Sri Ramachandra University Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soundararajan","family":"Periyasamy","sequence":"additional","affiliation":[{"name":"Department of Nephrology Sri Ramachandra University Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhaskar VKS","family":"Lakkakula","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Sri Ramachandra University Chennai India"},{"name":"Sickle Cell Institute Chhattisgarh Raipur India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2009020162"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.02190606"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000013300.11876.37"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2003.00264.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2005.03.035"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfg351"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1681\/ASN.V1181371","article-title":"Endothelium\u2010dependent relaxation of small resistance vessels is impaired in patients with autosomal dominant polycystic kidney disease","volume":"11","author":"Wang D","year":"2000","journal-title":"J. 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