{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T07:50:05Z","timestamp":1719906605089},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,12,23]],"date-time":"2014-12-23T00:00:00Z","timestamp":1419292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2015,1]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>Intrauterine growth retardation (IUGR<\/jats:styled-content>) can affect kidney development, leading to reduction in glomerular number. However, the associated cellular and molecular mechanisms have not been fully elucidated. This study investigated cell apoptosis and Bcl\u20102 and Bax expression in the kidney of IUGR<\/jats:styled-content> pups.<\/jats:p><\/jats:sec>Methods<\/jats:title>The IUGR<\/jats:styled-content> model was established in pregnant rats with 10% low\u2010protein diet. Renal cell apoptosis was detected using TUNEL<\/jats:styled-content> staining. Ki67<\/jats:styled-content> protein expression was measured by immunohistochemistry. Bcl\u20102 and Bax mRNA<\/jats:styled-content> expression was measured by real\u2010time polymerase chain reaction (PCR)<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>Significant decreases in the number of glomeruli was observed in the kidney of IUGR<\/jats:styled-content> pups 2 and 3 months after birth (P<\/jats:italic>\u2009<\u20090.01). Obvious apoptosis was observed in the kidney in both groups 1\u2009d, 7\u2009d, and 21\u2009d after birth with a peak at 7\u2009d. Significantly higher apoptosis index was observed in the kidney of IUGR<\/jats:styled-content> pups compared to control pups (P<\/jats:italic>\u2009<\u20090.01). No significant difference in proliferation was observed between the two groups. Significantly lower Bcl\u20102 mRNA<\/jats:styled-content> expression, Bcl\u20102\/Bax ratio, and higher Bax mRNA<\/jats:styled-content> expression were observed in IUGR<\/jats:styled-content> pups compared to control pups after birth (P<\/jats:italic>\u2009<\u20090.01).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The reduction in glomerular number in IUGR<\/jats:styled-content> pups is associated with increase in renal cell apoptosis. The reduction in Bcl\u20102\/Bax ratio may play a crucial role in renal cell apoptosis in IUGR<\/jats:styled-content> pups.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nep.12340","type":"journal-article","created":{"date-parts":[[2014,9,22]],"date-time":"2014-09-22T07:16:00Z","timestamp":1411370160000},"page":"34-39","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Apoptosis in the kidneys of rats that experienced intrauterine growth restriction"],"prefix":"10.1111","volume":"20","author":[{"given":"Xiaori","family":"He","sequence":"first","affiliation":[{"name":"Department of Neonatology Second Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zongde","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Neonatology Second Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingyi","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Neonatology Second Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pingyang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neonatology Second Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingtao","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Neonatology Second Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neonatology Second Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,12,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-6386(05)80006-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2003.270"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004670000560"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2004100875"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2010040352"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2011.09.015"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2010.553"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22497"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-011-1788-y"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00265.2005"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-010-0551-3"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040957"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00201.2003"},{"key":"e_1_2_6_15_1","first-page":"71","article-title":"Glomerular damage after uninephrectomy in young rats. I Hypertrophy and distortion of capillary architecture","volume":"42","author":"Nagata M","year":"2009","journal-title":"Kidney Int."},{"key":"e_1_2_6_16_1","first-page":"1601","article-title":"Apoptosis during an early stage of nephrogenesisinduces renal hypoplasia in bcl\u20102\u2010deficient mice","volume":"148","author":"Nagata M","year":"1996","journal-title":"Am. J. Pathol."},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1996.18"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/14767058.2012.733770"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/0886022X.2012.676526"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1681.2003.03787.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867321666140304094649"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2007.01809.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000094538"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-002-0262-9"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2009.24.1.138"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semnephrol.2007.02.007"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032771"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2006.02.009"}],"container-title":["Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnep.12340","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nep.12340","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T09:39:15Z","timestamp":1696153155000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nep.12340"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,23]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1111\/nep.12340"],"URL":"https:\/\/doi.org\/10.1111\/nep.12340","archive":["Portico"],"relation":{},"ISSN":["1320-5358","1440-1797"],"issn-type":[{"value":"1320-5358","type":"print"},{"value":"1440-1797","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,23]]},"assertion":[{"value":"2014-09-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}