{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T15:50:21Z","timestamp":1775577021945,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T00:00:00Z","timestamp":1450828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008713","name":"Diabetes Australia Research Trust","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008713","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Podocytes are specialized epithelial cells that play a critical role in the glomerular filtration barrier. Podocyte abnormalities are linked to increasing albuminuria and progression to end\u2010stage renal failure as observed in diabetic nephropathy. Advanced glycation end products (<jats:styled-content style=\"fixed-case\">AGEs<\/jats:styled-content>) have been strongly linked to the development of diabetic nephropathy, and we have previously shown that <jats:styled-content style=\"fixed-case\">AGEs<\/jats:styled-content> inhibit ezrin actions in proximal tubule cells. As ezrin plays a key role in controlling podocyte foot process integrity, the present study investigated the contribution of ezrin to <jats:styled-content style=\"fixed-case\">AGE<\/jats:styled-content>\u2010induced podocyte damage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Conditionally immortalized human podocytes were cultured with or without <jats:styled-content style=\"fixed-case\">AGEs<\/jats:styled-content>, and changes in morphology, protein expression and cell function were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fully differentiated podocytes had long finger\u2010like protrusions forming slit diaphragm structures. Immunofluorescence showed ezrin is mainly localized to the cell membrane with intense fluorescence on cellular protrusions. After <jats:styled-content style=\"fixed-case\">AGE\u2010BSA<\/jats:styled-content> treatment for 6 days, podocytes were rounder with fewer protrusions. Western blotting showed a time\u2010dependent reduction in ezrin levels compared with <jats:styled-content style=\"fixed-case\">BSA<\/jats:styled-content>\u2010treated cells. <jats:styled-content style=\"fixed-case\">AGE\u2010BSA<\/jats:styled-content> treatment reduced podocyte adhesion to fibronectin and inhibited migration. Overexpression of ezrin completely reversed <jats:styled-content style=\"fixed-case\">AGE\u2010BSA<\/jats:styled-content> inhibition of podocyte adhesion to fibronectin and partially reversed <jats:styled-content style=\"fixed-case\">AGE<\/jats:styled-content>\u2010induced inhibition of migration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results show a role for ezrin in <jats:styled-content style=\"fixed-case\">AGE<\/jats:styled-content>\u2010induced podocyte damage and suggest a new avenue for possible therapeutic intervention in diabetic nephropathy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nep.12526","type":"journal-article","created":{"date-parts":[[2015,6,2]],"date-time":"2015-06-02T04:59:52Z","timestamp":1433221192000},"page":"13-20","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Ezrin contributes to impaired podocyte migration and adhesion caused by advanced glycation end products"],"prefix":"10.1111","volume":"21","author":[{"given":"Elizabeth Anne","family":"McRobert","sequence":"first","affiliation":[{"name":"Department of Medicine (Alfred) Monash University  Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leon A","family":"Bach","sequence":"additional","affiliation":[{"name":"Department of Medicine (Alfred) Monash University  Melbourne Victoria Australia"},{"name":"Department of Endocrinology and Diabetes Alfred Hospital  Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,12,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00045.2011"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2010.08.015"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006010033"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.4161\/cam.5.2.15081"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI12539"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2005.031732"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M210433200"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005010051"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2012.08710.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2007.11.016"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V133630"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.112.1.111"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mjt.0000178769.52610.69"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000065100.17349.CA"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2014.03.002"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfn085"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117296"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2013.04.006"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1681\/ASN.V1281589","article-title":"The glomerular epithelial cell anti\u2010adhesin podocalyxin associates with the actin cytoskeleton through interactions with ezrin","volume":"12","author":"Orlando RA","year":"2001","journal-title":"J. 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