{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T04:21:03Z","timestamp":1792815663454,"version":"4.5.0"},"reference-count":16,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,6,22]],"date-time":"2016-06-22T00:00:00Z","timestamp":1466553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Anti\u2010neutrophil cytoplasmic antibody (ANCA) is associated with small\u2010vessel vasculitis particularly in the kidneys and can induce the formation of neutrophil extracellular traps (NETs) from primed neutrophils. Recently we have reported that the induction of NETs correlates with ANCA affinity for myeloperoxidase (MPO) and disease activity in patients with MPO\u2010ANCA\u2010associated microscopic polyangiitis. To investigate whether MPO\u2010ANCA affinity is associated with the formation of NETs <jats:italic>in vivo<\/jats:italic>, we examined the occurrence of NETs in the renal tissues of patients with MPO\u2010ANCA\u2010associated microscopic polyangiitis and ANCA affinity by double immunofluorescence staining for NET components of citrullinated histone, MPO and PAD4 and by ELISA competition with MPO, respectively. We divided 30 MPO\u2010ANCA\u2010associated microscopic polyangiitis patients into 2 groups based on their ANCA affinity levels (IC<jats:sub>50<\/jats:sub> for the high: 0.11\u2009\u00b1\u20090.04\u2009\u00b5g\/mL (Group1) and IC<jats:sub>50<\/jats:sub> for the low: 0.66\u2009\u00b1\u20090.24\u2009\u00b5g\/mL (Group2)). Group1 showed a higher Birmingham vasculitis activity score (15.6\u2009\u00b1\u20095.7) and 73% of the patients presented clinically with rapidly progressive glomerulonephritis and histologically with focal\/crescentic glomerulonephritis (GN). Group 2 showed a lower Birmingham vasculitis activity score (9.2\u2009\u00b1\u20094.9) and 73% of the patients presented clinically with chronic renal failure and histologically with mixed\/sclerotic GN. Group 1 showed a much higher occurrence of NETs than Group 2. Our findings indicate that ANCA affinity was associated with the <jats:italic>in vivo<\/jats:italic> formation of NETs, which might be involved in the pathophysiology of patients with MPO\u2010ANCA\u2010associated microscopic polyangiitis.<\/jats:p>","DOI":"10.1111\/nep.12736","type":"journal-article","created":{"date-parts":[[2016,2,2]],"date-time":"2016-02-02T08:07:59Z","timestamp":1454400479000},"page":"624-629","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Myeloperoxidase anti\u2010neutrophil cytoplasmic antibody affinity is associated with the formation of neutrophil extracellular traps in the kidney and vasculitis activity in myeloperoxidase anti\u2010neutrophil cytoplasmic antibody\u2010associated microscopic polyangiitis"],"prefix":"10.1111","volume":"21","author":[{"given":"Masaharu","family":"Yoshida","sequence":"first","affiliation":[{"name":"Department of Nephrology Tokyo Medical University Hachioji Medical Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muneharu","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Nephrology Tokyo Medical University Hachioji Medical Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyo","family":"Sudo","sequence":"additional","affiliation":[{"name":"Department of Nephrology Tokyo Medical University Hachioji Medical Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadasu","family":"Kojima","sequence":"additional","affiliation":[{"name":"Department of Nephrology Tokyo Medical University Hachioji Medical Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiro","family":"Tomiyasu","sequence":"additional","affiliation":[{"name":"Department of Nephrology Tokyo Medical University Hachioji Medical Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Department of Nephrology Tokyo Medical University Hachioji Medical Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Oda","sequence":"additional","affiliation":[{"name":"Department of Nephrology Tokyo Medical University Hachioji Medical Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiyuki","family":"Yamada","sequence":"additional","affiliation":[{"name":"Yokohama City University  Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2014.103"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-012-0598-2"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gft416"},{"issue":"52","key":"e_1_2_7_5_1","first-page":"S28","article-title":"Two types of myeloperoxidase antineutrophil cytoplasmic autoantibodies with a high affinity and a low affinity in small vessel vasculitis","volume":"27","author":"Yoshida M","year":"2009","journal-title":"Clin. 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Ther."}],"container-title":["Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnep.12736","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nep.12736","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/nep.12736","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nep.12736","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T14:48:44Z","timestamp":1696171724000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nep.12736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,22]]},"references-count":16,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["10.1111\/nep.12736"],"URL":"https:\/\/doi.org\/10.1111\/nep.12736","archive":["Portico"],"relation":{},"ISSN":["1320-5358","1440-1797"],"issn-type":[{"value":"1320-5358","type":"print"},{"value":"1440-1797","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,22]]},"assertion":[{"value":"2015-06-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-01-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-06-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}