{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T21:55:21Z","timestamp":1763070921045},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,6,15]],"date-time":"2017-06-15T00:00:00Z","timestamp":1497484800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Regulatory T cells (Treg) are important in mediating immune tolerance and outcomes of allotransplantation. CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup>CD39<jats:sup>+<\/jats:sup> co\u2010expression identifies memory Treg; CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>\u2212<\/jats:sup>CD39<jats:sup>+<\/jats:sup> memory T effectors. We sought to determine CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+\/\u2212<\/jats:sup>CD39<jats:sup>+<\/jats:sup> expression from the peripheral blood of patients with end stage renal failure, following transplantation and during episodes of acute cellular rejection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>CD4<jats:sup>+<\/jats:sup> T cells were isolated from peripheral blood leucocytes and analysed for CD25 and CD39 expression by flow cytometry. Treg suppressive function was measured by suppression of autologous effector T\u2010cell proliferation by Treg in co\u2010culture.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+\/\u2212<\/jats:sup>CD39<jats:sup>+<\/jats:sup> T\u2010cell subsets were tracked longitudinally in the peripheral blood of 17 patients following renal transplantation. Patients with acute T\u2010cell\u2010mediated rejection diagnosed on biopsy had reduced CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup>CD39<jats:sup>+<\/jats:sup> mTreg (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) and CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>\u2212<\/jats:sup>CD39<jats:sup>+<\/jats:sup> mTeff (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) cells compared with non\u2010rejecting patients. CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup>CD39<jats:sup>+<\/jats:sup> mTreg (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) and CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>\u2212<\/jats:sup>CD39<jats:sup>+<\/jats:sup> mTeff (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.057) were reduced in long\u2010term transplant patients (&gt;1\u00a0year) compared with non\u2010immunosuppressed controls. Interestingly, remaining CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup>CD39<jats:sup>+<\/jats:sup> mTreg in the stable transplant patients displayed more potent suppressive capacity compared with non\u2010immunosuppressed controls (83.2%\u00a0\u00b1\u00a03.1% <jats:italic>vs<\/jats:italic> 45.7%\u00a0\u00b1\u00a08.0%, nTeff:Treg ratio 8:1, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup>CD39<jats:sup>+<\/jats:sup> mTreg and CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>\u2212<\/jats:sup>CD39<jats:sup>+<\/jats:sup> mTeff in peripheral blood can be tracked in renal transplant patients. Acute cellular rejection was accompanied by reduced mTreg and mTeff. Determining changes in these T\u2010cell subsets may help to identify patients with, or at high risk of, renal allograft rejection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nep.12894","type":"journal-article","created":{"date-parts":[[2016,8,12]],"date-time":"2016-08-12T18:22:38Z","timestamp":1471026158000},"page":"505-512","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Evaluation of CD4<sup>+<\/sup>CD25<sup>+\/\u2212<\/sup>CD39<sup>+<\/sup> T\u2010cell populations in peripheral blood of patients following kidney transplantation and during acute allograft rejection"],"prefix":"10.1111","volume":"22","author":[{"given":"Jennifer L","family":"McRae","sequence":"first","affiliation":[{"name":"Immunology Research Centre  Parkville, Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanne SJ","family":"Chia","sequence":"additional","affiliation":[{"name":"Immunology Research 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