{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:48:50Z","timestamp":1790185730769,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,7,29]],"date-time":"2018-07-29T00:00:00Z","timestamp":1532822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81370701"],"award-info":[{"award-number":["81370701"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The growing number of patients suffering from chronic renal disease (CKD) is a challenge for the development of innovative therapies. Researchers have studied the therapeutic effects of cell therapy in acute kidney injury (AKI). However, the therapeutic effect of conditional medium (CM) in the CKD models have been rarely reported. Here, we examined the effects of umbilical cord derived\u2010mesenchymal stem cells (hUC\u2010MSCs) CM on renal fibrosis in a rat model of unilateral ureteral obstruction (UUO).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Animals were randomly divided into three groups: sham\u2010operated, UUO, UUO + CM. CM was administered via the left renal artery after total ligation of the left ureter. Rats were killed after 14 days of obstruction. Histological changes and oxidative stress parameters were assessed. Western blotting and immunohistochemistry analysis were used to measure epithelial\u2010mesenchymal transition (EMT) markers, including epithelial cadherin (E\u2010cadherin), \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA), tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), Collagen\u2010I, and transforming growth factor \u03b21 (TGF\u2010\u03b21). Proliferation and apoptosis of renal tubular epithelial cells (RTEs) were also measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HucMSC\u2010CM significantly reduced the levels of malondialdehyde (MDA) and reactive oxygen species (ROS), and increased the activity of glutathione (GSH) induced by UUO. Moreover, CM significantly reduced the expression of TGF\u2010\u03b21, \u03b1\u2010SMA, TNF\u2010\u03b1 and Collagen\u2010I in UUO kidney, promoted the proliferation of RTEs and inhibited its apoptosis. In addition, the increased expression of E\u2010cadherin also reflects the effective improvement of renal interstitial fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study shows that CM protects UUO\u2010induced kidney damage and therefore could be a potential tool to prevent CKD progression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nep.13099","type":"journal-article","created":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T20:06:35Z","timestamp":1498939595000},"page":"728-736","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Human umbilical cord\u2010derived mesenchymal stem cells conditioned medium attenuate interstitial fibrosis and stimulate the repair of tubular epithelial cells in an irreversible model of unilateral ureteral obstruction"],"prefix":"10.1111","volume":"23","author":[{"given":"Bo","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics Chongqing International Science and Technology Cooperation Center for Child Development and Disorders  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng\u2010Xia","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Chongqing Key Laboratory of Pediatrics Chongqing International Science and Technology Cooperation Center for Child Development and Disorders  Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics Chongqing International Science and Technology Cooperation Center for Child Development and Disorders  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics Chongqing International Science and Technology Cooperation Center for Child Development and Disorders  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geng","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da\u2010Wei","family":"He","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan\u2010Yuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Wake Forest Institute for Regenerative Medicine Wake Forest School of Medicine  Winston\u2010Salem NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De\u2010Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"},{"name":"Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics Chongqing International Science and Technology Cooperation Center for Child Development and Disorders  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang\u2010Hui","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Urology Children's Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000123691.46138.E2"},{"key":"e_1_2_7_3_1","first-page":"385","article-title":"Growth factors and renal regeneration","volume":"30","author":"Flaquer M","year":"2010","journal-title":"Nefrologia"},{"key":"e_1_2_7_4_1","first-page":"456","article-title":"Unilateral obstructive nephropathy in the rabbit. I. Early morphologic, physiologic, and histochemical changes","volume":"28","author":"Nagle RB","year":"1973","journal-title":"Lab. Invest."},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00045.2005"},{"key":"e_1_2_7_6_1","first-page":"167","article-title":"Correlations between renal cortical interstitial fibrosis, atrophy of the proximal tubules and impairment of the glomerular filtration rate","volume":"15","author":"Mackensen\u2010Haen S","year":"1981","journal-title":"Clin. Nephrol."},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00362.2001"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0795"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.214"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368915X687534"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000438545"},{"key":"e_1_2_7_12_1","first-page":"1017","article-title":"Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis\u2010supportive function and other potentials","volume":"91","author":"Lu L","year":"2006","journal-title":"Haematologica"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4076"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2015.02.023"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2008050513"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-11-96"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.3999"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00352.2013"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2008.06.009"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2010.06.022"},{"key":"e_1_2_7_21_1","article-title":"Therapeutic effects of human amniotic fluid\u2010derived stem cells on renal interstitial fibrosis in a murine model of unilateral ureteral obstruction","volume":"8","author":"Sun D","year":"2013","journal-title":"PLoS One"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200319270"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-0286"},{"key":"e_1_2_7_24_1","article-title":"Human embryonic mesenchymal stem cell\u2010derived conditioned medium rescues kidney function in rats with established chronic kidney disease","volume":"7","author":"Koppen A","year":"2012","journal-title":"PLoS One"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2007020140"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2009.12.003"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.112.114173"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfr015"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2008070798"}],"container-title":["Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnep.13099","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nep.13099","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T12:30:23Z","timestamp":1694953823000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nep.13099"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,29]]},"references-count":28,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["10.1111\/nep.13099"],"URL":"https:\/\/doi.org\/10.1111\/nep.13099","archive":["Portico"],"relation":{},"ISSN":["1320-5358","1440-1797"],"issn-type":[{"value":"1320-5358","type":"print"},{"value":"1440-1797","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,29]]},"assertion":[{"value":"2017-01-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-06-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}