{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:46:54Z","timestamp":1747187214772,"version":"3.40.5"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,1,29]],"date-time":"2019-01-29T00:00:00Z","timestamp":1548720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fund for Scientific and Mobile project from the Faculty of Medicine at Trakia University, Stara Zagora, Bulgaria","award":["5\/2013","6\/2015"],"award-info":[{"award-number":["5\/2013","6\/2015"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim of the study was to investigate whether the functional IL10\u20101082A\/G polymorphism exert a role in congenital anomalies of the kidney and urinary tract (CAKUT) in children. Also, the serum IL\u201010 and its association with genotype and renal parenchymal damage in CAKUT were explored.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In the current case\u2010control study, 134 paediatric cases of CAKUT and 382 unrelated controls were included. The genotyping of <jats:italic>IL10<\/jats:italic>\u20101082A\/G polymorphism was performed by amplification refractory mutation system\u2010PCR and IL\u201010 serum level was determined by ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Although, the genotype and allelic frequencies of <jats:italic>IL10<\/jats:italic>\u20101082 A\/G polymorphism in cases and controls were similar (\u03c7<jats:sup>2<\/jats:sup>\u2009=\u20090.459; <jats:italic>P<\/jats:italic>\u2009=\u20090.79 and \u03c7<jats:sup>2<\/jats:sup>\u2009=\u20090.426; <jats:italic>P<\/jats:italic>\u2009=\u20090.51, respectively), significant different genotype distribution between patients with or without parenchymal damage\/reduction was observed (\u03c7<jats:sup>2<\/jats:sup>\u2009=\u20096.9; <jats:italic>P<\/jats:italic>\u2009=\u20090.032). The GG\u2010genotype was more frequent in cases with renal parenchymal damage\/reduction compared to patients with preserved parenchyma (22% vs. 9%; OR\u2009=\u20092.987; 95% CI\u2009=\u20090.979\u20139.468; <jats:italic>P<\/jats:italic>\u2009=\u20090.031). On the contrary, the heterozygous genotype was less frequent among cases with parenchymal damage\/reduction compared to cases with preserved parenchyma (39% vs. 59%; OR\u2009=\u20090.453; 95% CI\u2009=\u20090.214\u20130.958; <jats:italic>P<\/jats:italic>\u2009=\u20090.024). Additionally, the serum IL\u201010 was significantly higher in CAKUT patients compared to age\u2010sex\u2010matched controls (median 11.98; IQR: 7.14\u201331.6 vs. 5.92; IQR: 4.68\u201314.8; <jats:italic>P<\/jats:italic>\u2009=\u20090.0057). Among carriers of GG\u2010genotype significantly higher IL\u201010 level was detected in cases with parenchymal damage\/reduction, than cases with preserved parenchyma (<jats:italic>P<\/jats:italic>\u2009=\u20090.028).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results suggested that the functional \u20101082A\/G polymorphism in <jats:italic>IL10<\/jats:italic> is associated with risk of renal parenchymal damage\/reduction rather than genetic predisposition to CAKUT. Additionally, our study supposes that immunoregulatory cytokine IL\u201010 might have a significant role in CAKUT.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nep.13229","type":"journal-article","created":{"date-parts":[[2018,1,30]],"date-time":"2018-01-30T11:02:44Z","timestamp":1517310164000},"page":"213-220","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Interleukin\u201010\u20101082<scp>A\/G<\/scp> polymorphism is associated with renal parenchymal damage in congenital anomalies of the kidney and urinary tract"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0028-7692","authenticated-orcid":false,"given":"Lyuba D","family":"Miteva","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Immunology and Medical Genetics Trakia University  Stara Zagora Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilya S","family":"Kostadinova","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Medical Faculty Trakia University  Stara Zagora Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Spaska A","family":"Stanilova","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunology and Medical Genetics Trakia University  Stara Zagora Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3523"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c120028"},{"key":"e_1_2_7_4_1","article-title":"Interactions between cytokines, congenital anomalies of kidney and urinary tract and chronic kidney disease","volume":"2013","author":"Sim\u00f5es de Silva AC","year":"2013","journal-title":"Clin. 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