{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T03:56:32Z","timestamp":1770609392936,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,5,2]],"date-time":"2019-05-02T00:00:00Z","timestamp":1556755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Science & Technology Research Foundation of Yang Zhou City, Jiang Su, China","award":["SQZ201430047"],"award-info":[{"award-number":["SQZ201430047"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Klotho G\u2010395\u2010A gene polymorphism is associated with several diseases; however, its association with calcium\u2010phosphate metabolism disorders in end\u2010stage renal disease (ESRD) is unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 137 patients with ESRD and 80 healthy adults (control) were enrolled in the study. Patients with ESRD were divided into three subgroups: haemodialysis (A1, <jats:italic>n<\/jats:italic> =\u200952), peritoneal dialysis (A2, <jats:italic>n<\/jats:italic> =\u200930), and non\u2010dialysis (A3, <jats:italic>n<\/jats:italic> =\u200955). The klotho G\u2010395\u2010A genotype was detected by TaqMan PCR assay, and ELISA was used to detect the soluble klotho protein (sKL) and fibroblast growth factor (FGF23). Intact parathyroid hormone (iPTH) and other related clinical biochemical parameters were also analyzed for all subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>(i) Three genotypes (GG, GA and AA) of KL G\u2010395A were detected, and a significant difference between the ESRD and control groups was observed, (ii) sKL was inversely associated with FGF23 in each subgroup and phosphate and positively associated with calcium in A1 and A3. FGF23 was positively associated with phosphate and inversely associated with calcium in each subgroup, (iii) a statistical difference in levels of sKL and FGF23 was observed between GG and AA, as well as between GA and AA. The expression of sKL was lowest and the level of FGF23 was highest in AA and (iv). GA\u2009+\u2009AA genotypes and FGF23 were risk factors and sKL might be protective factor of calcium\u2010phosphate metabolism disorders.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Soluble klotho protein and FGF23 were associated with the regulation of calcium and phosphate metabolism, and the A allele of the G\u2010395A klotho gene polymorphism could be a risk factor on calcium\u2010phosphate metabolism disorders in patients with ESRD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nep.13547","type":"journal-article","created":{"date-parts":[[2018,12,8]],"date-time":"2018-12-08T08:23:50Z","timestamp":1544257430000},"page":"1001-1008","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Association of klotho gene polymorphism and the regulation of calcium\u2010phosphate metabolism disorders in patients with end\u2010stage renal disease"],"prefix":"10.1111","volume":"24","author":[{"given":"Qing\u2010Ya","family":"Zeng","sequence":"first","affiliation":[{"name":"Dalian Medical University  Dalian China"},{"name":"Nephrology Department Northern Jiangsu People's Hospitial  Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Yin","family":"Xia","sequence":"additional","affiliation":[{"name":"Nephrology Department The Affiliated Hospital of Yangzhou University  Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010Shan","family":"Tong","sequence":"additional","affiliation":[{"name":"Nephrology Department Northern Jiangsu People's Hospitial  Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Sun","sequence":"additional","affiliation":[{"name":"Nephrology Department Northern Jiangsu People's Hospitial  Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Bin","family":"Mou","sequence":"additional","affiliation":[{"name":"Nephrology Department Northern Jiangsu People's Hospitial  Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Chen","sequence":"additional","affiliation":[{"name":"Nephrology Department Northern Jiangsu People's Hospitial  Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang\u2010Yu","family":"Bi","sequence":"additional","affiliation":[{"name":"Nephrology Department Northern Jiangsu People's Hospitial  Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang\u2010Hua","family":"Liu","sequence":"additional","affiliation":[{"name":"Nephrology Department Northern Jiangsu People's Hospitial  Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000343408"},{"key":"e_1_2_7_3_1","first-page":"1582","article-title":"Polymorphism of klotho G\u2010395A and susceptibility of coronary artery disease in East\u2010Asia population: A meta\u2010analysis","volume":"8","author":"Zhai C","year":"2015","journal-title":"Int. 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