{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:20:20Z","timestamp":1790630420984,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T00:00:00Z","timestamp":1554336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>This study was conducted to investigate the chronic injury of peritoneal glucose injection on the peritoneum and intestine and the protective effects of omega\u20103 polyunsaturated fatty acid (\u03c9\u20103PUFA) during peritoneal dialysis (PD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peritoneal dialysis animal models were established by intraperitoneal injection of 4.25% glucose for 28\u2009days. Protein expression in ileum and peritoneum was measured by immunofloresence and immunohistochemistry. Protein expression in macrophages was measured by Western blot. Fibrosis was analyzed by Masson staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Peritoneal dialysis significantly increased the structural injury and decreased junction\u2010related protein ZO\u20101 and occludin expression in ileum, the expression of proteins relating to the activation of M2 (Erg2, IRF4), but not M1 (CD38, IRF5) macrophages. PD significantly increased the expression of TGF\u2010\u03b21, VEGF and ALK5 protein in peritoneal tissues. PD significantly increased fibrosis (Masson staining) and the expression of fibroblast marker \u03b1\u2010SMA in peritoneal tissues. Injection of macrophage clean reagent and \u03c9\u20103PUFA significantly inhibited M2 activation, and decreased Masson staining, \u03b1\u2010SMA, TGF\u2010\u03b21, VEGF and ALK5 protein expression in peritoneal tissues in PD treated rats. \u03c9\u20103PUFA injection significantly decreased PD\u2010induced injury in ileum and normalized the expression of ZO\u20101 and occludin in the ileum of PD rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Omega\u20103 fatty acids can provide a protective role on PD\u2010induced peritoneal fibrosis and injury of the intestine.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nep.13587","type":"journal-article","created":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T01:11:39Z","timestamp":1553044299000},"page":"1081-1089","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Protective effects and mechanisms of omega\u20103 polyunsaturated fatty acid on intestinal injury and macrophage polarization in peritoneal dialysis rats"],"prefix":"10.1111","volume":"24","author":[{"given":"Hanfen","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Nutrition The Second Xiangya Hospital, Central South University  Changsha China"},{"name":"Institute of Nephrology The Second Xiangya Hospital, Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuping","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Nutrition The Second Xiangya Hospital, Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cai","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Nutrition The Second Xiangya Hospital, Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Nutrition The Second Xiangya Hospital, Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8104-5101","authenticated-orcid":false,"given":"Fuyou","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Nephrology The Second Xiangya Hospital, Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-011-0533-y"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2016.03.040"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2018.03.014"},{"key":"e_1_2_7_5_1","first-page":"002","article-title":"The mechanism of peritoneal fibrosis in peritoneal dialysis","volume":"3","author":"Onishi A","year":"2011","journal-title":"J. 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